PXD052417

PXD052417 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Heloderma horridum horridum venom
Description	Heloderma horridum horridum commonly known as the scorpion lizard, Mexican scorpion, and beaded lizard, is a venomous reptile native of America. The venom derived from this lizard has potential applications, particularly in treatment of type II diabetes through the peptide Exendin. In this work, H. h. horridum venom was extracted from adult specimens and lyophilized. To characterize the venom, enzymatic assays, including hyaluronidase, phospholipase A2, and proteolytic activity were conducted. A proteomic analysis of the venom was also performed employing bottom-up/shotgun approaches from SDS-PAGE and High pH Reversed-Phase chromatography., besides fractionation of tryptic peptides using a nano-LC-MS/MS. These approaches involved massive sequencing to enhancing the likelihood of detecting an extensive range of venom proteins. The proteins and peptides found in H. h. horridum venom are reviewed according to the classification of the transcriptome previously reported.
HostingRepository	PRIDE
AnnounceDate	2024-08-11
AnnouncementXML	Submission_2024-08-10_16:07:47.729.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052417
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Miguel Elizalde
SpeciesList	scientific name: Heloderma horridum horridum; NCBI TaxID: 8552;
ModificationList	monohydroxylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 16:27:07	ID requested
⏵ 1	2024-08-10 16:07:48	announced

10.1021/acs.jproteome.4c00287;

Lino-L, ó, pez GJ, Ruiz-May E, Elizalde-Contreras JM, Jim, é, nez-Vargas JM, Rodr, í, guez-V, á, zquez A, Gonz, á, lez-Carrillo G, Boj, ó, rquez-Vel, á, zquez E, Garc, í, a-Villalvazo PE, Berm, ú, dez-Guzm, á, n MJ, Zatarain-Palacios R, V, á, zquez-Vuelvas OF, Valdez-Vel, á, zquez LL, Corzo G, Venom: Assessment to Its Transcriptome and Newfound Proteins. J Proteome Res, 23(8):3638-3648(2024) [pubmed]

10.6019/PXD052417;

submitter keyword: Lizard, Proteome, Enzymatic activities, Venom

Eliel Ruiz-May
contact affiliation	Instituto de Ecología, Carretera antigua a Coatepec 351, El Haya, Xalapa, Veracruz, México.
contact email	eliel.ruiz@inecol.mx
lab head
Miguel Elizalde
contact affiliation	Instituto de Ecolog'ia A.C.
contact email	jose.elizalde@inecol.mx
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD052417

PRIDE project URI

• PRIDE
  1. PXD052417
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Heloderma horridum horridum venom