PXD052414

PXD052414 is an original dataset announced via ProteomeXchange.

Title	Kinetic redox proteomic analysis reveals distinct lipopolysaccharide effects, limited by a manganese Superoxide Dismutase mimic
Description	Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammatory bowel disease (IBD) pathogenesis. A kinetic redox proteomic strategy (OcSILAC) was used to explore the lipopolysaccharide (LPS) effect, together with the role of the Mn1 SOD-mimic, in the intestinal HT29-MD2 cellular model. Cysteine oxidation was the main early event of LPS treatment, attenuated over 6 h, as counteracted by rapid response systems on both protein levels (SOD1, NQO1, ALDH1) and post-translational levels (oxidized peroxiredoxins), and a late preponderant increased SOD2 level. Mn1 exerted a potent antioxidant effect, limiting the LPS-induced oxidation and increasing antioxidant enzyme levels, and a variable anti-inflammatory effect attenuating the level of most of the LPS-regulated proteins. Our results reveal the multifaceted LPS effect in intestinal epithelial cells (IECs) and highlight Mn1 as a potential effective anti-oxidant and anti-inflammatory agent for IBD treatment, and a useful tool to explore the oxidation and inflammation interconnection.
HostingRepository	PRIDE
AnnounceDate	2025-03-05
AnnouncementXML	Submission_2025-03-04_23:52:18.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Chiappetta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 14:03:06	ID requested
⏵ 1	2025-03-04 23:52:19	announced

10.1002/ANIE.202422644.;

submitter keyword: LPS, manganese, oxidation,cysteine, ROS

Joelle Vinh
contact affiliation	Lab's head
contact email	joelle.vinh@espci.fr
lab head
Giovanni Chiappetta
contact affiliation	ESPCI-CNRS
contact email	giovanni.chiappetta@espci.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052414
     1. Label: PRIDE project
     2. Name: Kinetic redox proteomic analysis reveals distinct lipopolysaccharide effects, limited by a manganese Superoxide Dismutase mimic