PXD052401

PXD052401 is an original dataset announced via ProteomeXchange.

Title	The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein
Description	Microalgae are promising production platforms for the cost-effective production of recombinant proteins. We have recently established that the red alga Porphyridium purpureum provides superior transgene expression properties, due to the episomal main- tenance of transformation vectors as multicopy plasmids in the nucleus. Here, we have explored the potential of Porphyridium to synthesize complex pharmaceutical proteins to high levels. Testing expression constructs for a candidate subunit vaccine against the hepatitis C virus (HCV), we show that the soluble HCV E2 glycoprotein can be produced in transgenic algal cultures to high levels. The antigen undergoes faithful posttranslational modification by N-glycosylation and is recognized by conformationally selective antibodies, suggesting that it adopts a proper antigenic conformation in the endoplasmic reticulum of red algal cells. We also report the experimental determina- tion of the structure of the N-glycan moiety that is attached to glycosylated proteins in Porphyridium. Finally, we demonstrate the immunogenicity of the HCV antigen produced in red algae when administered by injection as pure protein or by feeding of algal biomass.
HostingRepository	PRIDE
AnnounceDate	2024-05-21
AnnouncementXML	Submission_2024-05-21_03:36:18.417.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lara Hoepfner
SpeciesList	scientific name: Porphyridium purpureum; NCBI TaxID: 35688;
ModificationList	complex glycosylation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 02:46:39	ID requested
⏵ 1	2024-05-21 03:36:18	announced

Dataset with its publication pending

submitter keyword: N-glycosylation,HCV, Porphyridium

Michael Hippler
contact affiliation	Institute for plant biology and biotechnology, University of Münster
contact email	mhippler@uni-muenster.de
lab head
Lara Hoepfner
contact affiliation	IBBP-Plant Biochemistry and Biotechnology AG Prof. Dr. Michael Hippler University of Münster
contact email	l_hoep03@wwu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052401
     1. Label: PRIDE project
     2. Name: The red alga Porphyridium as a host for molecular farming: Efficient production of immunologically active hepatitis C virus glycoprotein