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PXD052398

PXD052398 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFGFR2 activation by FGF2 stimulation and FGFR2 fusion mutations or FGFR2 inhibition by Pemigatinib stimulation in cholangiocarcinoma cell lines, followed by detection of changes in WDR5 binding proteins
DescriptionThe human bile duct cancer cell line RBE was subjected to serum starvation for 8 hours in serum-free medium, including both FGFR2 knockout and wild-type conditions, followed by co-incubation with FGF2 and Pemigatinnb. Cell lysis was conducted using RIPA buffer supplemented with protease and phosphatase inhibitors. The cell lysates were sonicated and centrifuged at 12,000 × g for 15 minutes at 4°C to extract total proteins, and the supernatant containing protein lysates was collected. During this process, magnetic beads pre-bound with WDR5 antibody (AB307664, abcam) and conjugated with agarose (HY-K0202, MCE) were mixed according to the instructions and incubated at room temperature on a rotator for 30 minutes. The beads were washed five times with washing buffer and then mixed with the above-mentioned supernatant, followed by overnight incubation on a rotator at 4°C with gentle shaking at 12 revolutions per minute. After washing, the beads were collected, and the proteins adsorbed on the beads were released by heating at 95°C for 5 minutes. Subsequently, 10% SDS-PAGE gel electrophoresis was performed. For conventional protein mass spectrometry analysis, all gel bands were excised.
HostingRepositoryiProX
AnnounceDate2024-05-17
AnnouncementXMLSubmission_2025-05-15_19:45:46.983.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYan Song
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-20 00:10:25ID requested
12025-05-15 19:45:47announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: CCA,FGFR2,FGF2,Fusion,WDR5
Contact List
Zongli Zhang
contact affiliationShandong University Qilu Hospital
contact emailzzlzzl1900@163.com
lab head
Yan Song
contact affiliationShandong University Qilu Hospital
contact emailSongymedical@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI