PXD052398
PXD052398 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | FGFR2 activation by FGF2 stimulation and FGFR2 fusion mutations or FGFR2 inhibition by Pemigatinib stimulation in cholangiocarcinoma cell lines, followed by detection of changes in WDR5 binding proteins |
| Description | The human bile duct cancer cell line RBE was subjected to serum starvation for 8 hours in serum-free medium, including both FGFR2 knockout and wild-type conditions, followed by co-incubation with FGF2 and Pemigatinnb. Cell lysis was conducted using RIPA buffer supplemented with protease and phosphatase inhibitors. The cell lysates were sonicated and centrifuged at 12,000 × g for 15 minutes at 4°C to extract total proteins, and the supernatant containing protein lysates was collected. During this process, magnetic beads pre-bound with WDR5 antibody (AB307664, abcam) and conjugated with agarose (HY-K0202, MCE) were mixed according to the instructions and incubated at room temperature on a rotator for 30 minutes. The beads were washed five times with washing buffer and then mixed with the above-mentioned supernatant, followed by overnight incubation on a rotator at 4°C with gentle shaking at 12 revolutions per minute. After washing, the beads were collected, and the proteins adsorbed on the beads were released by heating at 95°C for 5 minutes. Subsequently, 10% SDS-PAGE gel electrophoresis was performed. For conventional protein mass spectrometry analysis, all gel bands were excised. |
| HostingRepository | iProX |
| AnnounceDate | 2024-05-17 |
| AnnouncementXML | Submission_2025-05-15_19:45:46.983.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yan Song |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-05-20 00:10:25 | ID requested | |
| ⏵ 1 | 2025-05-15 19:45:47 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CCA,FGFR2,FGF2,Fusion,WDR5 |
Contact List
| Zongli Zhang | |
|---|---|
| contact affiliation | Shandong University Qilu Hospital |
| contact email | zzlzzl1900@163.com |
| lab head | |
| Yan Song | |
| contact affiliation | Shandong University Qilu Hospital |
| contact email | Songymedical@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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