PXD052397

PXD052397 is an original dataset announced via ProteomeXchange.

Title	In cholangiocarcinoma, WDR5 modification changes are promoted by using FGF2 stimulation or constructing FGFR2 fusion mutations, or by using inhibitors to inhibit FGFR2 function
Description	The human bile duct cancer cell line RBE was subjected to serum starvation for 8 hours in serum-free medium, including both FGFR2 knockout and wild-type conditions, followed by co-incubation with FGF2 and Pemigatinnb. Cell lysis was conducted using RIPA buffer supplemented with protease and phosphatase inhibitors. The cell lysates were sonicated and centrifuged at 12,000 × g for 15 minutes at 4°C to extract total proteins, and the supernatant containing protein lysates was collected. During this process, magnetic beads pre-bound with WDR5 antibody (AB307664, abcam) and conjugated with agarose (HY-K0202, MCE) were mixed according to the instructions and incubated at room temperature on a rotator for 30 minutes. The beads were washed five times with washing buffer and then mixed with the above-mentioned supernatant, followed by overnight incubation on a rotator at 4°C with gentle shaking at 12 revolutions per minute. After washing, the beads were collected, and the proteins adsorbed on the beads were released by heating at 95°C for 5 minutes. Subsequently, 10% SDS-PAGE gel electrophoresis was performed. For conventional protein mass spectrometry analysis, all gel bands were excised.
HostingRepository	iProX
AnnounceDate	2024-05-28
AnnouncementXML	Submission_2025-05-15_19:44:24.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Song
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-20 00:07:51	ID requested
⏵ 1	2025-05-15 19:44:24	announced

Dataset with its publication pending

submitter keyword: RBE,FGFR2,WDR5,Modification,Phosphorylation

Zongli Zhang
contact affiliation	Shandong University Qilu Hospital
contact email	zzlzzl1900@163.com
lab head
Yan Song
contact affiliation	Shandong University Qilu Hospital
contact email	Songymedical@163.com
dataset submitter

iProX dataset URI