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PXD052372

PXD052372 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlecircMAN1A2_009-pulled down proteins in HeLa cells
DescriptionCircRNA pulldown assays were conducted to identify proteins interacting with circMAN1A2_009. In summary, HeLa cell protein extracts were incubated with biotin-labeled circMAN1A2_009 probes obtained from Tsingke Biotechnology, China, at room temperature for 1h. Subsequently, streptavidin magnetic beads were introduced into the cell lysates containing the probes and incubated overnight at 4°C. After three washes, proteins bound to the streptavidin magnetic beads were eluted using the Elution Buffer as per the instruction provided by the PierceTM Magnetic RNA-Protein Pull-Down Kit (Thermo, 20164, USA). The retrieved proteins underwent analysis through liquid chromatography-tandem mass spectrometry (LS-MS/MS) conducted by OE biotech Co. Ltd (Shanghai, China), or Western Blot.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_17:01:27.856.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXinyi Wei
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-19 05:04:11ID requested
12025-05-06 17:01:28announced
Publication List
Huang Y, Wei X, Tu M, Lu W, Xu J, CircMAN1A2_009 facilitates YBX1 nuclear localization to induce GLO1 activation for cervical adenocarcinoma cell growth. Cancer Sci, 115(10):3273-3287(2024) [pubmed]
10.1111/cas.16264;
Keyword List
submitter keyword: circMAN1A2_009, HeLa, pull-down assays
Contact List
Junfen Xu
contact affiliationDepartment of Gynecologic Oncology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang, China
contact emailxjfzu@zju.edu.cn
lab head
Xinyi Wei
contact affiliationzhejiang university
contact emailweixinyi@zju.edu.cn
dataset submitter
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Dataset FTP location
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PRIDE project URI
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