PXD052372 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | circMAN1A2_009-pulled down proteins in HeLa cells |
Description | CircRNA pulldown assays were conducted to identify proteins interacting with circMAN1A2_009. In summary, HeLa cell protein extracts were incubated with biotin-labeled circMAN1A2_009 probes obtained from Tsingke Biotechnology, China, at room temperature for 1h. Subsequently, streptavidin magnetic beads were introduced into the cell lysates containing the probes and incubated overnight at 4°C. After three washes, proteins bound to the streptavidin magnetic beads were eluted using the Elution Buffer as per the instruction provided by the PierceTM Magnetic RNA-Protein Pull-Down Kit (Thermo, 20164, USA). The retrieved proteins underwent analysis through liquid chromatography-tandem mass spectrometry (LS-MS/MS) conducted by OE biotech Co. Ltd (Shanghai, China), or Western Blot. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-06_17:01:27.856.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xinyi Wei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-19 05:04:11 | ID requested | |
⏵ 1 | 2025-05-06 17:01:28 | announced | |
Publication List
Huang Y, Wei X, Tu M, Lu W, Xu J, CircMAN1A2_009 facilitates YBX1 nuclear localization to induce GLO1 activation for cervical adenocarcinoma cell growth. Cancer Sci, 115(10):3273-3287(2024) [pubmed] |
10.1111/cas.16264; |
Keyword List
submitter keyword: circMAN1A2_009, HeLa, pull-down assays |
Contact List
Junfen Xu |
contact affiliation | Department of Gynecologic Oncology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, Zhejiang, China |
contact email | xjfzu@zju.edu.cn |
lab head | |
Xinyi Wei |
contact affiliation | zhejiang university |
contact email | weixinyi@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052372
- Label: PRIDE project
- Name: circMAN1A2_009-pulled down proteins in HeLa cells