PXD052362

PXD052362 is an original dataset announced via ProteomeXchange.

Title	Multimodal cell maps as a foundation for structural and functional genomics
Description	Human cells contain a complex hierarchy of components, many of which remain uncharted. Here we broadly identify subcellular components across the 10–9–10–5 meter range, via self-supervised fusion of immunofluorescence images and biophysical interactions measured for >5100 proteins in U2-OS human cells. This global multi-scale map resolves 275 molecular assemblies, which we systematically validate by whole-cell size-exclusion chromatography and annotate using large language models. We explore key applications in driving 3D structural biology, yielding novel structures for 79 heterodimeric complexes and an expanded 10-protein Rag-Ragulator assembly. The map assigns functions to 46 understudied proteins, including a role for C18orf21 in RNA processing. It functionally decodes cancer genomes, identifying 21 recurrently mutated assemblies including cell junctions, SWI/SNF complexes, and an NCOR complex, encompassing 102 cancer driver proteins that validate by transposon mutagenesis. The Multiscale Integrated Cell portal provides a queryable reference platform for structural and functional cell biology.
HostingRepository	PRIDE
AnnounceDate	2025-01-08
AnnouncementXML	Submission_2025-01-08_12:07:11.552.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-18 05:25:59	ID requested
⏵ 1	2025-01-08 12:07:11	announced

Dataset with its publication pending

submitter keyword: size-exclusion chromatography,Protein-correlation profiling, LCMSMS

Leonard J. Foster
contact affiliation	Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD052362

PRIDE project URI

• PRIDE
  1. PXD052362
     1. Label: PRIDE project
     2. Name: Multimodal cell maps as a foundation for structural and functional genomics