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PXD052356

PXD052356 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBrain tissue Protein Profile of Periplaneta americana exposed to commercial pesticide
DescriptionVarious organic and natural pesticides play a vital role in insect control, leading to variation in their protein profile (Abubakar et al., 2019; James & Xu, 2012). The sex, size, weight, and growth conditions could be the possible factors associated with fluctuations in the concentration of protein production in the cockroach (Bouchebtiet al., 2022) The current study of Perplanata americana under pesticide activity considered to observe the proteomics of Brain and protein expression under stressed conditions. 15 cockroaches were exposed to 5g of Laxman Rekha (11:1 ratio) to assess the impact of pesticide on protein synthesis. National and international research conducted on aqueous and alcoholic extraction of gut, exoskeleton and brain protein of Periplaneta spp. and Blatta spp have revealed 10 potential antibacterial proteins between 18 kDa to 72kDa range. These proteins were purified using chromatographic techniques like single dimension reducing PAGE, FPLC-MS, LC-MS, MALDI TOF-MS etc. The current study is aimed at isolation, identification and characterisation of proteins from cockroach brain tissue lysate using Liquid chromatography and Orbitrap Mass spectrometer technique. The data analysis was done using Thermo Proteome Discoverer software version 2.2. 0.388. The database used was Uniprot-Periplaneta americana.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:47:37.857.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD052356
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSiddharth Sagar
SpeciesList scientific name: Periplaneta americana; NCBI TaxID: 6978;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-17 11:04:47ID requested
12024-06-27 01:57:12announced
22024-10-22 06:47:38announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD052356;
Keyword List
submitter keyword: Pesticide, Brain Tissue, LC-MS/MS
Contact List
Dr.Jayaprada Rao
contact affiliationDepartment of Biotechnology,Mithibai College,India
contact emailjayaprada_chunduri@hotmail.com
lab head
Siddharth Sagar
contact affiliationCo-founder and Professor at Prarambh Science Academy, Sangli
contact emailsiddharth.sgr@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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