PXD052336

PXD052336 is an original dataset announced via ProteomeXchange.

Title	Shared proteomic and transcriptomic signatures of molecularly-induced feedback and feedforward wiring of adult hippocampal neurons
Description	Molecular programming by basic helix-loop-helix (bHLH) proteins can potently reactivate axon growth in adult neurons, enabling the investigation of the mechanisms and the potentials of adult brain rewiring. Here, we focused on the mechanisms by studying proteomic and transcriptomic changes during Id2- (a bHLH transcriptional regulator) and Ascl4- (a bHLH transcription factor) induced feedback and feedforward wiring of adult hippocampal granule cells. Despite these differences, we find that Id2 and Ascl4 share a molecular cascade through the transcription factor Stat1 and co-regulate a set of proteins, which potentially constitute a core program for adult wiring. Unexpectedly, we also identify shared molecular changes which do not directly originate from the reprogrammed neurons, but from endothelial cells and microglia. Together, our results indicate that adult brain wiring is regulated by specific molecular programs and dynamic interactions between the wiring neurons and the circuit environment.
HostingRepository	PRIDE
AnnounceDate	2026-01-28
AnnouncementXML	Submission_2026-01-28_10:55:24.219.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Charlotte Seng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; formylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-17 02:11:32	ID requested
⏵ 1	2026-01-28 10:55:24	announced

Dataset with its publication pending

submitter keyword: dentate gyrus, rewiring, Id2, Ascl4

Csaba Földy
contact affiliation	Brain Research Institute, University of Zurich
contact email	foldy@hifo.uzh.ch
lab head
Charlotte Seng
contact affiliation	University of Zurich, Brain Research Institute
contact email	seng@hifo.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD052336

PRIDE project URI

• PRIDE
  1. PXD052336
     1. Label: PRIDE project
     2. Name: Shared proteomic and transcriptomic signatures of molecularly-induced feedback and feedforward wiring of adult hippocampal neurons