PXD052318

PXD052318 is an original dataset announced via ProteomeXchange.

Title	Modulation of Intracellular Pathways of HIV-1 infected Macrophages exposed to Sigma-1 Receptor Antagonist (BD1047) prior to Cocaine and its Role in Cathepsin B Secretion revealed by Quantitative Proteomics
Description	HIV-1 infects monocytes derived macrophages (MDM), that migrate into the brain, and secrete neurotoxic molecules, including cathepsin B (CATB). Cocaine potentiates CATB secretion. Pretreatment with BD1047, a sigma-1 receptor antagonist, before cocaine exposure, reduces infection, CATB secretion, and neuronal apoptosis in vitro. We aim to elucidate intracellular pathways dysregulated after pretreatment with BD1047 versus those exposed to cocaine only, using Tandem Mass Tag Proteomics (TMT). Significant criteria of (|≥1.5| fold change, p-values ≤0.05) were used. Results demonstrate that pretreatment with BD1047 prior to cocaine shared six (6) proteins in comparison with the cocaine group that pertain to ubiquitination, ribosomal activity, and cell morphology. BD1047 uniquely dysregulated eighty (80) proteins when compared to infected cocaine group; and fifteen (15) of those were selected by Ingenuity Pathways analyses and literature review regarding infection, CATB and mitochondrial dysfunction. BD1047 pretreatment upregulated proteins related to oxidative phosphorylation activation (SLC25A-31), mitochondrial dysfunction (ATP5PD), ion transport dysfunction (VDAC2-3), endoplasmic reticulum transport hyperactivity (PHB, TMED10, CANX), and cytoskeleton remodeling (TUB1A-C, ANXA1). Similarly, downregulated proteins conveyed to Golgi transport (SURF4) and cell cytoskeleton proteins (PLEC, OLA1, GFAP). BD1047 uniquely dysregulates independent proteins from cocaine that might act as protective and balancing mechanisms for reducing mitochondrial damage, infection and CATB secretion.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_16:33:30.175.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052318
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Loyda Melendez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 07:54:29	ID requested
⏵ 1	2025-05-06 16:33:32	announced

10.6019/PXD052318;

10.3390/biomedicines12091934;

V, é, lez-L, ó, pez O, Carrasquillo-Carri, ó, n K, Cantres-Rosario YM, Mach, í, n-Mart, í, nez E, Á, lvarez-R, í, os ME, Roche-Lima A, Tosado-Rodr, í, guez EL, Mel, é, ndez LM, Analysis of Sigma-1 Receptor Antagonist BD1047 Effect on Upregulating Proteins in HIV-1-Infected Macrophages Exposed to Cocaine Using Quantitative Proteomics. Biomedicines, 12(9):(2024) [pubmed]

submitter keyword: monocyte-derived macrophages (MDM), BD1047,Human immunodeficiency virus (HIV), cocaine

Loyda M Melendez
contact affiliation	University of Puerto RIco, Medical Sciences Campus, Translational Proteomics Center
contact email	lmelendezlab@gmail.com
lab head
Loyda Melendez
contact affiliation	University of Puerto Rico Medical Sciences Campus
contact email	lmelendezlab@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052318

PRIDE project URI

• PRIDE
  1. PXD052318
     1. Label: PRIDE project
     2. Name: Modulation of Intracellular Pathways of HIV-1 infected Macrophages exposed to Sigma-1 Receptor Antagonist (BD1047) prior to Cocaine and its Role in Cathepsin B Secretion revealed by Quantitative Proteomics