PXD052316 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TRAF6 substrate profiling by Ub-BOD |
Description | Our method (Ub-POD) exploits the proximity and the relative orientation of the E3-ligase catalytic domain with respect to ubiquitin observed in the enzymatic intermediate-state structures of E3-E2~Ub. By fusing biotin ligase BirA and an Avi tag variant to the candidate E3 ligase and ubiquitin, respectively, we were able to specifically enrich the bona fide substrates and potentially new substrates of the ligase using a one-step Streptavidin pulldown under denaturing conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_05:07:10.364.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Behrends |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-16 06:54:06 | ID requested | |
⏵ 1 | 2024-10-17 05:09:23 | announced | |
Publication List
10.1126/sciadv.adp3000; |
Mukhopadhyay U, Levantovsky S, Carusone TM, Gharbi S, Stein F, Behrends C, Bhogaraju S, A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates. Sci Adv, 10(32):eadp3000(2024) [pubmed] |
Keyword List
submitter keyword: HeLa, BirA, Ubiquitin |
Contact List
Christian Behrends |
contact affiliation | Ludwig-Maximilians-Universität München |
contact email | christian.behrends@mail03.med.uni-muenchen.de |
lab head | |
Christian Behrends |
contact affiliation | SyNergy, LMU München |
contact email | christian.behrends@mail03.med.uni-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD052316 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052316
- Label: PRIDE project
- Name: TRAF6 substrate profiling by Ub-BOD