PXD052316

PXD052316 is an original dataset announced via ProteomeXchange.

Title	TRAF6 substrate profiling by Ub-BOD
Description	Our method (Ub-POD) exploits the proximity and the relative orientation of the E3-ligase catalytic domain with respect to ubiquitin observed in the enzymatic intermediate-state structures of E3-E2~Ub. By fusing biotin ligase BirA and an Avi tag variant to the candidate E3 ligase and ubiquitin, respectively, we were able to specifically enrich the bona fide substrates and potentially new substrates of the ligase using a one-step Streptavidin pulldown under denaturing conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:07:10.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Behrends
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 06:54:06	ID requested
⏵ 1	2024-10-17 05:09:23	announced

10.1126/sciadv.adp3000;

Mukhopadhyay U, Levantovsky S, Carusone TM, Gharbi S, Stein F, Behrends C, Bhogaraju S, A ubiquitin-specific, proximity-based labeling approach for the identification of ubiquitin ligase substrates. Sci Adv, 10(32):eadp3000(2024) [pubmed]

submitter keyword: HeLa, BirA, Ubiquitin

Christian Behrends
contact affiliation	Ludwig-Maximilians-Universität München
contact email	christian.behrends@mail03.med.uni-muenchen.de
lab head
Christian Behrends
contact affiliation	SyNergy, LMU München
contact email	christian.behrends@mail03.med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD052316

PRIDE project URI

• PRIDE
  1. PXD052316
     1. Label: PRIDE project
     2. Name: TRAF6 substrate profiling by Ub-BOD