PXD052311

PXD052311 is an original dataset announced via ProteomeXchange.

Title	Transcriptional and proteomic dynamics of severe fever with thrombocytopenia syndrome virus infection in Rhipicephalus microplus cells identify antiviral host factors.
Description	Ticks are vectors of arboviruses in many parts of the world. The rising incidence and emergence of tick-borne arboviral infections across human populations indicates that further transmission control strategies including those based on vectors, will be required to reduce the burden of disease. However, arbovirus-tick interactions at the cellular level remain poorly understood in general, and particularly neglected for negative strand RNA arboviruses. In this study we developed a proteomics informed by transcriptomics approach to characterize the cellular response of Rhipicephalus microplus-derived cell cultures to infection with the tick-borne pathogen severe fever with thrombocytopenia syndrome virus (SFTSV, Phenuiviridae). For this, we generated the first de novo transcriptomes and confirmed proteomes of SFTSV- or mock-infected tick cell cultures derived from a vector species that transmits the virus in nature. Through comprehensive annotation of genes, proteins and pathway analysis, we identified core host responses and regulatory processes mediated in response to SFTSV infection. Moreover, examining the interactome of the virally encoded nucleoprotein (N) allowed us to integrate host responses with the analysis of cellular factors required for viral replication. The influence of specific host genes on SFTSV replication was systematically assessed through dsRNA-mediated gene silencing. This functional genomics approach pinpointed two tick-derived RNA helicases as critical antiviral factors capable of restricting SFTSV infection: the DexD/box helicase (DHX9) and the Up-Frameshift Protein 1 (UPF1). Collectively, our findings enrich the repository of resources available for understanding the antiviral response to SFTSV infection in Rh. microplus vector cells and support the identification of SFTSV-antiviral restrictions factors.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-26_00:24:44.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Rhipicephalus microplus; NCBI TaxID: 6941; scientific name: Bunyavirales; NCBI TaxID: NCBITaxon:1980410; scientific name: Severe fever with thrombocytopenia syndrome virus; NCBI TaxID: 1003835;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:39:34	ID requested
⏵ 1	2025-05-26 00:24:45	announced

Petit MJ, Flory C, Gu Q, Fares M, Lamont D, Score A, Davies K, Bell-Sakyi L, Scaturro P, Brennan B, Kohl A, Multi-omics analysis of SFTS virus infection in Rhipicephalus microplus cells reveals antiviral tick factors. Nat Commun, 16(1):4732(2025) [pubmed]

10.1038/s41467-025-59565-w;

submitter keyword: SFTSV, AP-MS/MS,Rhipicephalus microplus, severe fever with thrombocytopenia syndrome

Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology (LIV), Hamburg, Germany
contact email	pietro.scaturro@leibniz-liv.de
lab head
Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052311

PRIDE project URI

• PRIDE
  1. PXD052311
     1. Label: PRIDE project
     2. Name: Transcriptional and proteomic dynamics of severe fever with thrombocytopenia syndrome virus infection in Rhipicephalus microplus cells identify antiviral host factors.