PXD052310

PXD052310 is an original dataset announced via ProteomeXchange.

Title	A proteomic and functional view of intrabacterial lipid inclusion biogenesis in mycobacteria
Description	During infection and granuloma formation, pathogenic mycobacteria accumulate triacylglycerol in the form of Intrabacterial Lipid Inclusions (ILI). These organelles facilitate bacterial evasion from the immune system and serve as a carbon source to sustain bacterial persistence within the infected host. M. abscessus, a rapidly-growing non-tuberculous opportunistic pathogen, was previously shown to generate similar structures throughout their infectious cycle. Herein, it was used as a model organism to identify, at a global level, the ILI-associated proteins (IAP) involved in ILI accumulation. By implementing the APEX2 proximity labeling method using an in vitro model for ILI accumulation, we identified 228 proteins potentially implicated in ILI biosynthesis.
HostingRepository	PRIDE
AnnounceDate	2025-02-27
AnnouncementXML	Submission_2025-02-27_11:44:23.178.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AUDEBERT Stephane
SpeciesList	scientific name: Mycobacterium tuberculosis; NCBI TaxID: NCBITaxon:1773; scientific name: Mycobacterium abscessus; NCBI TaxID: 36809;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:39:02	ID requested
⏵ 1	2025-02-27 11:44:24	announced

10.1128/mbio.01475-24;

Dargham T, Aguilera-Correa JJ, Avellan R, Mallick I, Celik L, Santucci P, Brasseur G, Poncin I, Point V, Audebert S, Camoin L, Daher W, Cavalier J-F, Kremer L, Canaan S, A proteomic and functional view of intrabacterial lipid inclusion biogenesis in mycobacteria. mBio, 16(4):e0147524(2025) [pubmed]

submitter keyword: LC-MSMS,Intracellular lipid inclusion, lipid metabolism, APEX2, persistence, ILI-associated proteins, proteomic, Mycobacterium abscessus, proximity labelling

Stéphane Canaan, Ph. D
contact affiliation	Stéphane Canaan, Ph. D , Group Leader, "Lipolysis and Bacterial Pathogenicity" Laboratory of Engineering of Macromolecular Systems UMR7255-CNRS, 31 Chemin Joseph-Aiguier 13402 MARSEILLE cedex 20
contact email	Stephane.canaan@imm.cnrs.fr
lab head
AUDEBERT Stephane
contact affiliation	Marseille Proteomic, Centre de Recherche en Cancérologie de Marseille, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes, 27 Boulevard Leï Roure CS30059 13273 Marseille Cedex 09 France
contact email	stephane.audebert@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052310
     1. Label: PRIDE project
     2. Name: A proteomic and functional view of intrabacterial lipid inclusion biogenesis in mycobacteria