PXD052307 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ribosome-inactivation by a widely distributed class of C-tail anchored membrane proteins |
Description | Ribosome hibernation is a commonly used strategy that protects ribosomes under unfavorable conditions and regulates developmental processes. Multiple ribosome-hibernation factors have been identified in all domains of life, but due to their structural diversity and the lack of a common mechanism by which they inactivate ribosomes, it is currently unknown how many different hibernation factors exist. Here, we demonstrate that the YqjD/ElaB/YgaM protein family constitute an abundant class of ribosome-hibernating proteins and represent the first membrane-bound hibernating factors identified in bacteria. Our data demonstrate that they interact with 50S ribosomal subunit and show a concentration-dependent inhibition of protein synthesis. By combining in vivo cross-linking with mass spectrometry, we show that they bind via their N-termini to proteins that surround the ribosomal tunnel exit and even penetrate into the ribosomal tunnel. Thus, YqjD/ElaB/YgaM inhibit translation by blocking the ribosomal tunnel and thus mimic the activity of antimicrobial peptides and macrolide antibiotics. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-04 |
AnnouncementXML | Submission_2025-03-04_05:50:30.948.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD052307 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Friedel Drepper |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-16 05:34:09 | ID requested | |
⏵ 1 | 2025-03-04 05:50:31 | announced | |
Publication List
Keyword List
submitter keyword: translation |
stress response, YgaM, macrolide antibiotics,YqjD, ElaB, antimicrobial peptides |
Contact List
Friedel Drepper |
contact affiliation | Faculty of Biology, University of Freiburg, Freiburg, Germany |
contact email | f.drepper@biologie.uni-freiburg.de |
lab head | |
Friedel Drepper |
contact affiliation | University of Freiburg |
contact email | f.drepper@biologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD052307 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052307
- Label: PRIDE project
- Name: Ribosome-inactivation by a widely distributed class of C-tail anchored membrane proteins