PXD052306

PXD052306 is an original dataset announced via ProteomeXchange.

Title	Fatty Acid Synthase as Interacting Anticancer Target of the Terpenoid Myrianthic Acid disclosed by MS-Based Proteomics Approaches
Description	This research focuses on the target deconvolution of the natural compound myrianthic acid, a triterpenoid characterized by an ursane skeleton isolated from the roots of Myrianthus arboreus and from Oenothera maritima Nutt. (Onagraceae), using MS-based chemical proteomic techniques. Application of Drug Affinity Responsive Target Stability (DARTS) and targeted-Limited Proteolysis coupled to Mass Spectrometry (t-LiP-MS) led to the identification of the enzyme fatty acid synthase (FAS) as an interesting macromolecular counterpart of myrianthic acid. This result, confirmed by comparison with the natural triteprenoid ursolic acid, was thoroughly investigated and validated in silico by molecular docking, which gave a precise picture of the interactions in the MA/FAS complex. Moreover, in vitro biological assays showcased the inhibitory activity of myrianthic acid against the FAS enzyme, most likely related to its antiproliferative activity towards tumor cells. Given the sig-nificance of FAS in specific pathologies, especially cancer, the myrianthic acid structural moieties could serve as a promising reference point to start a potential development of innovative ap-proaches in therapy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:10.136.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gilda D'Urso
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:33:41	ID requested
1	2024-06-23 02:09:58	announced
⏵ 2	2024-10-22 06:47:11	announced	2024-10-22: Updated project metadata.

10.3390/ijms25115918;

Capuano A, D'Urso G, Gazzillo E, Lauro G, Chini MG, D'Auria MV, Ferraro MG, Iazzetti F, Irace C, Bifulco G, Casapullo A, Fatty Acid Synthase as Interacting Anticancer Target of the Terpenoid Myrianthic Acid Disclosed by MS-Based Proteomics Approaches. Int J Mol Sci, 25(11):(2024) [pubmed]

submitter keyword: drug discovery

functional proteomics

fatty acid synthase

t-LiP

DARTS

molecular docking

pre-clinical investigations

Agostino Casapullo
contact affiliation	Department of Pharmacy, University of Salerno
contact email	casapullo@unisa.it
lab head
Gilda D'Urso
contact affiliation	University of Salerno
contact email	gidurso@unisa.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052306
     1. Label: PRIDE project
     2. Name: Fatty Acid Synthase as Interacting Anticancer Target of the Terpenoid Myrianthic Acid disclosed by MS-Based Proteomics Approaches