PXD052297

PXD052297 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum PfEMP1 DiQ-BioID data
Description	PfEMP1 is the major virulence factor of Plasmodium falciparum parasites. PfEMP1 is expressed as an integral membrane protein on the surface of infected red blood cells. As a part of this project, the BioID system was utilized to biotinylate proximal proteins of a functional PfEMP1. In order to do this, selection linked integration (SLI) was used to modify a specific PfEMP1 (PfIT_060021400; IT4var01) by fusing it with the promiscuous biotin ligase BirA* and obtain a parasite population predominantly expressing this modified variant. Three separate IT4 cell lines were generated where BirA* was fused to different positions of PfEMP1. In the first cell line (Position 1), BirA* is C-terminal to the ATS domain of PfEMP1, in the second cell line (position 2), BirA* is between the ATS and the transmembrane domain and in the third cell line (position 3), BirA* is between the transmembrane and the extracellular binding domains. Biotinylated proteins extracted with either triton or SDS containing buffers (using sequential extraction of the same parasite pellet) were isolated in large scales and subsequently analyzed via mass spectrometry in three separate experiments for the three BioID cell line as well as for IT4 control parasites (parent). The experiments resulted in a set of enriched proteins for each of the 3 cell lines (position 1-3) over IT4 parasites.
HostingRepository	PRIDE
AnnounceDate	2025-11-13
AnnouncementXML	Submission_2025-11-13_06:29:04.953.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pascal Jansen
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: NEWT:5833;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:21:59	ID requested
⏵ 1	2025-11-13 06:29:05	announced

Dataset with its publication pending

submitter keyword: var genes, virulence, protein export,Malaria, PfEMP1, BirA*, selection linked integration, BioID, Plasmodium falciparum

Tobias Spielmann
contact affiliation	Malaria Cell Biology. Bernhard Nocht Institute for Tropical Medicine. Hamburg, Germany
contact email	spielmann@bnitm.de
lab head
Pascal Jansen
contact affiliation	Radboud University
contact email	p.jansen@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD052297

PRIDE project URI

• PRIDE
  1. PXD052297
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum PfEMP1 DiQ-BioID data