PXD052292 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of human macrophages overexpressing angiotensin-converting enzyme |
Description | Angiotensin converting enzyme (ACE) exerts strong modulation of myeloid cell function independently of its cardiovascular arm. The success of the ACE-overexpressing murine macrophage model, ACE 10/10, in treating microbial infections and cancer opens a new venture into whether ACE overexpression in human macrophages share these benefits. Additionally, as ACE inhibitors are a widely used antihypertensive medication, their impact on ACE expressing immune cells is of interest and currently understudied. In the present study, we utilized mass spectrometry to characterize and assess global proteomic changes in an ACE overexpressing human THP-1 cell line. Additional proteomic and cellular uptake data following treatment with an ACE C-domain selective inhibitor, lisinopril-tryptophan, was also obtained. ACE activity was significantly decreased with inhibitor treatment despite limited uptake within the cell and both RNA processing and immune pathways were significantly dysregulated with treatment. Also present were up-regulated energy and TCA cycle, cytokine and interleukin signaling proteins with ACE overexpression. A novel immune pathway that appeared both with ACE overexpression and inhibitor treatment was neutrophil degranulation. ACE overexpression within human macrophages shows similarities with ACE 10/10 murine macrophages paving the way for mechanistic studies into understanding the altered immune function. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-09 |
AnnouncementXML | Submission_2025-06-08_16:07:19.132.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tariq Ganief |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-16 05:19:31 | ID requested | |
⏵ 1 | 2025-06-08 16:07:20 | announced | |
Publication List
Oosthuizen D, Ganief TA, Bernstein KE, Sturrock ED, Proteomic Analysis of Human Macrophages Overexpressing Angiotensin-Converting Enzyme. Int J Mol Sci, 25(13):(2024) [pubmed] |
10.3390/ijms25137055; |
Keyword List
submitter keyword: ACE, SWATH, protein inhibitor, Angiotensin, protein function |
Contact List
Edward Sturrock |
contact affiliation | Division of Chemical, Systems and Synthetic Biology, Institute for Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town |
contact email | edward.sturrock@uct.ac.za |
lab head | |
Tariq Ganief |
contact affiliation | Dr |
contact email | taganief@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD052292 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052292
- Label: PRIDE project
- Name: Proteomic analysis of human macrophages overexpressing angiotensin-converting enzyme