PXD052292

PXD052292 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human macrophages overexpressing angiotensin-converting enzyme
Description	Angiotensin converting enzyme (ACE) exerts strong modulation of myeloid cell function independently of its cardiovascular arm. The success of the ACE-overexpressing murine macrophage model, ACE 10/10, in treating microbial infections and cancer opens a new venture into whether ACE overexpression in human macrophages share these benefits. Additionally, as ACE inhibitors are a widely used antihypertensive medication, their impact on ACE expressing immune cells is of interest and currently understudied. In the present study, we utilized mass spectrometry to characterize and assess global proteomic changes in an ACE overexpressing human THP-1 cell line. Additional proteomic and cellular uptake data following treatment with an ACE C-domain selective inhibitor, lisinopril-tryptophan, was also obtained. ACE activity was significantly decreased with inhibitor treatment despite limited uptake within the cell and both RNA processing and immune pathways were significantly dysregulated with treatment. Also present were up-regulated energy and TCA cycle, cytokine and interleukin signaling proteins with ACE overexpression. A novel immune pathway that appeared both with ACE overexpression and inhibitor treatment was neutrophil degranulation. ACE overexpression within human macrophages shows similarities with ACE 10/10 murine macrophages paving the way for mechanistic studies into understanding the altered immune function.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_16:07:19.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tariq Ganief
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:19:31	ID requested
⏵ 1	2025-06-08 16:07:20	announced

Oosthuizen D, Ganief TA, Bernstein KE, Sturrock ED, Proteomic Analysis of Human Macrophages Overexpressing Angiotensin-Converting Enzyme. Int J Mol Sci, 25(13):(2024) [pubmed]

10.3390/ijms25137055;

submitter keyword: ACE, SWATH, protein inhibitor, Angiotensin, protein function

Edward Sturrock
contact affiliation	Division of Chemical, Systems and Synthetic Biology, Institute for Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town
contact email	edward.sturrock@uct.ac.za
lab head
Tariq Ganief
contact affiliation	Dr
contact email	taganief@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052292
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human macrophages overexpressing angiotensin-converting enzyme