PXD052289

PXD052289 is an original dataset announced via ProteomeXchange.

Title	Canid alphaherpesvirus 1 infection affects the gene expression and secretome profile of canine adipose-derived mesenchymal stem cells in vitro
Description	Canine adipose -derived mesenchymal stem cells (cAD-MSCs) demonstrate promising tissue repair and regeneration capabilities. However, the procurement and preservation of these cells or their secreted factors for therapeutic applications pose a risk of viral contamination, and the consequences on cAD-MSCs remain unexplored. Consequently, this research sought to assess the impact of Canid alphaherpesvirus 1 on the functional attributes of cAD-MSCs, including gene expression profiles and secretome composition. To this end, abdominal fat tissue from twelve healthy dogs was harvested to isolate cAD-MSCs. These samples were tested for CHV contamination before introducing a wild-type CHV strain via serial passages. Following CHV infection, RT-PCR arrays and LC-MS/MS assessments enabled gene expression analysis and the secretome's proteomic landscape, respectively. The study established that the initial cAD-MSCs populations were devoid of CHV. Upon exposure to the virus, cAD-MSCs exhibited susceptibility, leading to notable modifications in gene expression and secretome profile. These changes reflected their intrinsic properties, such as stemness, differentiation potential, structural integrity, proliferative capacity, survival, directional migration, and immune-modulatory functions, depriving their regenerative efficacy . Despite the absence of pre-existing contamination, the outcomes underscore the imperative of routine viral screening prior to therapeutic use, particularly CHV. Moreover, these findings provide insights into the pathogenic mechanisms of CHV and the intricate interactions between canine stem cells and viruses.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-16_03:43:46.482.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052289
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marina Prišlin
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-16 05:18:17	ID requested
⏵ 1	2024-12-16 03:43:46	announced

10.6019/PXD052289;

10.1186/S12985-024-02603-8;

submitter keyword: Mesenchymal stem cell

Canine adipose-derived mesenchymal stem cell

In vitro

Gene expression

Secretome

Orthoherpesviridae

Canid alphaherpesvirus 1

In vitro infection

Veterinary regenerative medicine

Dragan Brnić
contact affiliation	Principal investigator of HRZZ-UIP-2019-04-2178 SECRET project
contact email	brnic@veinst.hr
lab head
Marina Prišlin
contact affiliation	Virology Department, Croatian Veterinary Institute, Savska cesta 143, 10000 Zagreb
contact email	prislin.marina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD052289

PRIDE project URI

• PRIDE
  1. PXD052289
     1. Label: PRIDE project
     2. Name: Canid alphaherpesvirus 1 infection affects the gene expression and secretome profile of canine adipose-derived mesenchymal stem cells in vitro