PXD052286 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Activity-Based Protein Profiling identifies an alpha-amylase family protein contributing to the virulence of Methicillin-resistant Staphylococcus aureus |
| Description | Glycosidases fulfill crucial functions in all life forms contributing to diverse biological processes such as metabolism, cell signaling, or pathogen-host interactions. In search for new putative targets for antimicrobial drugs, we here aimed to identify and characterize retaining glycosidase activities in the bacterial pathogen Staphylococcus aureus. We initially screened the activities of diverse retaining glycosidase families in S. aureus JE2 under different growth conditions using panel of seven different fluorescent activity-based probes in a gel-based system, before using a pool of three related biotinylated probes for target enrichment and identification by mass-spectrometry-based activity-based protein profiling. Three glycoside hydrolase family proteins were identified: 6-phospho-β-glucosidase (BglA), α-amylase family protein trehalase C (TreC), and autolysin (Atl). The physiological relevance of the previously uncharacterized glycosidases BglA and TreC were addressed using CRISPRi-induced gene silencing. Whereas both enzymes were dispensable for in vitro growth in Tryptic Soy Broth, silencing of treC, but not bglA, led to reduced virulence in a Galleria mellonella infection model, warranting future investigations into the biochemical function and physiological role of this enzyme. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-05-07 |
| AnnouncementXML | Submission_2025-05-06_16:32:44.950.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Md Jalal Uddin |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-05-16 05:17:08 | ID requested | |
| ⏵ 1 | 2025-05-06 16:32:45 | announced | |
Publication List
Keyword List
| submitter keyword: chemoproteomics · activity-based probe · biofilm· virulence |
Contact List
| Christian S. Lentz |
|---|
| contact affiliation | Department of Medical Biology, UiT- The Arctic University of Norway, 9019 Tromsø, Norway |
| contact email | christian.s.lentz@uit.no |
| lab head | |
| Md Jalal Uddin |
|---|
| contact affiliation | UiT The Arctic University of Norway |
| contact email | jalal.uddin@uit.no |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052286
- Label: PRIDE project
- Name: Activity-Based Protein Profiling identifies an alpha-amylase family protein contributing to the virulence of Methicillin-resistant Staphylococcus aureus
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