PXD052259

PXD052259 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of BRAFi-treated endothelial cells
Description	Targeted therapies against mutant BRAF are effectively used in combination with MEK inhibitors (MEKi) to treat advanced melanoma. However, treatment success is affected by resistance and adverse events (AEs). Approved BRAF inhibitors (BRAFi) show high levels of target promiscuity, which can contribute to these effects. Blood vessels are in direct contact with high plasma concentrations of BRAFi, but effects of the inhibitors in this cell type are unknown. Hence, we aimed to characterize responses to clinically relevant BRAF kinase inhibitors in the vascular endothelium. A mass spectrometry-based analysis of the phosphoproteome of dermal microvascular endothelial cells revealed distinct sets of off-targets of the used BRAFi. Meanwhile, there were only negligible changes to the proteome after 1 h of treatment. Together with our published functional data, these findings provide insights on the surprisingly distinct side effects of BRAFi on endothelial signaling and functionality.
HostingRepository	PRIDE
AnnounceDate	2024-06-01
AnnouncementXML	Submission_2024-06-01_03:37:55.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sophie Bromberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-14 05:43:43	ID requested
⏵ 1	2024-06-01 03:37:55	announced

Dataset with its publication pending

submitter keyword: Human, Phosphoproteomics, Endothelial cells, BRAFi, Vemurafenib

Klaudia Schossleitner
contact affiliation	Department of Dermatology, Medical University of Vienna
contact email	klaudia.schossleitner@muv.ac.at
lab head
Sophie Bromberger
contact affiliation	Department of Dermatology, Medical University of Vienna
contact email	sophie.bromberger@muv.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD052259

PRIDE project URI

• PRIDE
  1. PXD052259
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of BRAFi-treated endothelial cells