PXD052258

PXD052258 is an original dataset announced via ProteomeXchange.

Title	Protein composition of the extracellular matrix from bovine ovarian tissue before and after decellularization
Description	Recent approaches of regenerative reproductive medicine investigate the decellularized extracellular matrix to develop a transplantable engineered ovary (TEO). However, a full proteomic analysis is not usually performed after the decellularization process to evaluate the preservation of the extracellular matrix (ECM). In this study, the ECM of the bovine ovarian cortex was analyzed before and after decellularization using mass spectrometry and bioinformatics. A total of 155 matrisome proteins were identified in the native ECM of the bovine ovarian cortex, with 145 matrisome proteins detected in the decellularized ECM. After decellularization, only 10 matrisome proteins were lost, and notably, none belonged to the category of reproductive biological processes. Histology and histochemistry analyses were employed to assess the general morphology of both native and decellularized ECM, allowing for the identification of the most abundant ECM proteins. Moreover, our study highlighted collagen type VI alpha 3 and heparan sulfate proteoglycan 2 as the most abundant components in the bovine ovarian ECM, mirroring the composition observed in the human ovary. These findings enhance our understanding of the composition of both native and decellularized ECM, with the potential implications for the development of a TEO.
HostingRepository	PRIDE
AnnounceDate	2024-11-15
AnnouncementXML	Submission_2024-11-15_04:12:44.719.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052258
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-14 05:21:38	ID requested
⏵ 1	2024-11-15 04:12:45	announced

10.1016/J.JPROT.2024.105347;

10.6019/PXD052258;

submitter keyword: ECM, proteomics, proteins,Matrisome, ovary

Christiani Andrade Amorim
contact affiliation	UCLOuvain, Pôle de Recherche en Physiopathologie de la Reproduction, Avenue Hippocrate, 54 Bte. B1.55.03 - 1200 Bruxelles, Belgique
contact email	christiani.amorim@uclouvain.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052258
     1. Label: PRIDE project
     2. Name: Protein composition of the extracellular matrix from bovine ovarian tissue before and after decellularization