PXD052246

PXD052246 is an original dataset announced via ProteomeXchange.

Title	Isolation, Molecular Characterisation, and Immunomodulatory Effects of Extracellular Vesicles From Spirulina Microalgae
Description	SPEVs (Spirulina EV) were isolated through a combination of techniques involving ultracentrifugation and size exclusion chromatography. Cryo-Transmission Electron Microscopy revealed the presenc of pleomorphic outer-membrane-vesicles (OMVs) and outer-inner-membrane-vesicles (OIMVs) displaying diverse shapes, sizes, and corona densities. High-resolution liquid chromatography-mass spectrometry was performed on SPEVs utilising both gel-based and in-solution techniques for sample processing. We identified a total of 54 proteins derived from SPEVs, with a few of the proteins with the highest “unused protein algorithmic score” t belonging to the bacterial porin superfamily.
HostingRepository	PRIDE
AnnounceDate	2024-12-19
AnnouncementXML	Submission_2024-12-19_02:14:49.711.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yide Wong
SpeciesList	scientific name: Limnospira maxima; NCBI TaxID: 129910; scientific name: Arthrospira platensis; NCBI TaxID: 118562;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 23:53:57	ID requested
⏵ 1	2024-12-19 02:14:50	announced

10.1002/jex2.70025;

Sharifpour MF, Sikder S, Wong Y, Koifman N, Thomas T, Courtney R, Seymour J, Loukas A, Characterization of Spirulina-derived extracellular vesicles and their potential as a vaccine adjuvant. J Extracell Biol, 3(12):e70025(2024) [pubmed]

submitter keyword: Spirulina, Extracellular Vesicles, Proteomics

Yide Wong
contact affiliation	Australian Institute of Tropical Health and Medicine. Centre for Molecular Therapeutics. James Cook Univerisity, Cairns.
contact email	alex.loukas@jcu.edu.au
lab head
Yide Wong
contact affiliation	James Cook University
contact email	yide.wong@jcu.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD052246

PRIDE project URI

• PRIDE
  1. PXD052246
     1. Label: PRIDE project
     2. Name: Isolation, Molecular Characterisation, and Immunomodulatory Effects of Extracellular Vesicles From Spirulina Microalgae