PXD052244 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomic analysis of the role of knocking down NRP1 after OGD/R in primary renal tubular epithelial cells |
Description | Neuropilin-1 (NRP1), a co-receptor for various cytokines, including TGF-β, has been identified as a potential therapeutic target for fibrosis. However, its role and mechanism in renal fibrosis remains elusive. Here, we show that NRP1 is upregulated in distal tubular (DT) cells of patients with transplant renal insufficiency and mice with renal ischemia-reperfusion (I-R) injury. Knockout of Nrp1 reduced multiple endpoints of renal injury and fibrosis. We found that Nrp1 facilitates the binding of TNF-α to its receptor in DT cells after renal injury. This signaling results in a downregulation of lysine crotonylation of the metabolic enzyme Cox4i1, decreased cellular energetics and exacerbation of renal injury. Furthermore, by single-cell RNA-sequencing we found that Nrp1-positive DT cells secrete collagen and communicate with myofibroblasts, exacerbating acute kidney injury (AKI)-induced renal fibrosis by activating Smad3. Dual genetic deletion of Nrp1 and Tgfbr1 in DT cells better improves renal injury and fibrosis than either single knockout. Together, these results reveal that targeting of NRP1 represents a promising strategy for the treatment of AKI and subsequent chronic kidney disease |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-03 |
AnnouncementXML | Submission_2024-06-03_03:53:25.916.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yinzheng Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-05-13 21:26:17 | ID requested | |
⏵ 1 | 2024-06-03 03:53:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: lysine crotonylation,NRP1, chronic kidney disease, acute kidney injury, aerobic metabolism |
Contact List
Rui Zeng |
contact affiliation | Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology |
contact email | lyzhengmz@163.com |
lab head | |
Yinzheng Li |
contact affiliation | Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology |
contact email | lyzhengmz@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD052244 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052244
- Label: PRIDE project
- Name: Quantitative proteomic analysis of the role of knocking down NRP1 after OGD/R in primary renal tubular epithelial cells