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PXD052244

PXD052244 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic analysis of the role of knocking down NRP1 after OGD/R in primary renal tubular epithelial cells
DescriptionNeuropilin-1 (NRP1), a co-receptor for various cytokines, including TGF-β, has been identified as a potential therapeutic target for fibrosis. However, its role and mechanism in renal fibrosis remains elusive. Here, we show that NRP1 is upregulated in distal tubular (DT) cells of patients with transplant renal insufficiency and mice with renal ischemia-reperfusion (I-R) injury. Knockout of Nrp1 reduced multiple endpoints of renal injury and fibrosis. We found that Nrp1 facilitates the binding of TNF-α to its receptor in DT cells after renal injury. This signaling results in a downregulation of lysine crotonylation of the metabolic enzyme Cox4i1, decreased cellular energetics and exacerbation of renal injury. Furthermore, by single-cell RNA-sequencing we found that Nrp1-positive DT cells secrete collagen and communicate with myofibroblasts, exacerbating acute kidney injury (AKI)-induced renal fibrosis by activating Smad3. Dual genetic deletion of Nrp1 and Tgfbr1 in DT cells better improves renal injury and fibrosis than either single knockout. Together, these results reveal that targeting of NRP1 represents a promising strategy for the treatment of AKI and subsequent chronic kidney disease
HostingRepositoryPRIDE
AnnounceDate2024-06-03
AnnouncementXMLSubmission_2024-06-03_03:53:25.916.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYinzheng Li
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-05-13 21:26:17ID requested
12024-06-03 03:53:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: lysine crotonylation,NRP1, chronic kidney disease, acute kidney injury, aerobic metabolism
Contact List
Rui Zeng
contact affiliationDivision of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact emaillyzhengmz@163.com
lab head
Yinzheng Li
contact affiliationDivision of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact emaillyzhengmz@163.com
dataset submitter
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Dataset FTP location
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