PXD052232

PXD052232 is an original dataset announced via ProteomeXchange.

Title	ALBA proteins facilitate cytoplasmic YTHDF-mediated reading of m6A in plants
Description	N6-methyladenosine (m6A) exerts many of its regulatory effects on eukaryotic mRNAs by recruiting cytoplasmic YT521-B homology domain family (YTHDF) proteins. Here, we show that in Arabidopsis, the interaction between m6A and the major YTHDF protein ECT2 also involves the mRNA-binding ALBA protein family. ALBA and YTHDF proteins physically associate via a deeply conserved short linear motif in the intrinsically disordered region of YTHDF proteins, their mRNA targets overlap, and ALBA4 binding sites are juxtaposed to m6A sites. These binding sites correspond to pyrimidine-rich elements previously found to be important for m6A binding of ECT2. Accordingly, both biological effects of ECT2 and its binding to m6A targets in vivo require ALBA association. Our results introduce the YTHDF-ALBA complex as the functional cytoplasmic m6A-reader in plants and define a molecular foundation for the concept of facilitated m6A reading that increases the potential for combinatorial control of biological m6A effects.
HostingRepository	PRIDE
AnnounceDate	2024-11-18
AnnouncementXML	Submission_2024-11-18_12:01:22.428.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mathias Tankmar
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 11:38:00	ID requested
⏵ 1	2024-11-18 12:01:22	announced

10.1038/S44318-024-00312-0;

submitter keyword: Arabidopsis,YTHDF, m6A, ALBA

Peter Brodersen
contact affiliation	Professor at Section for Computational and RNA Biology, Copenhagen University
contact email	pbrodersen@bio.ku.dk
lab head
Mathias Tankmar
contact affiliation	Copenhagen University
contact email	mathias.tankmar@bio.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052232
     1. Label: PRIDE project
     2. Name: ALBA proteins facilitate cytoplasmic YTHDF-mediated reading of m6A in plants