PXD052226

PXD052226 is an original dataset announced via ProteomeXchange.

Title	Influence of Albumin Concentration on Surface Characteristics and Cellular Responses in the Pre-Incubation of Multi-Walled Carbon Nanotubes
Description	Due to the characteristics of multi-walled carbon nanotubes (MWCNTs) to aggregate and bind non-specifically, attempts have been made to inhibit their aggregation and increase their dispersion stability for biomedical applications by coating with bovine serum albumin (BSA). However, BSA concentration has not been studied in detail in terms of cellular response. To investigate the surface proteins formed in 10% fetal bovine serum cell culture media depending on the BSA concentration used to stabilize MWCNTs and to correlate the cellular responses with BSA concentration, we performed nanoflow liquid chromatography–electrospray ionization–tandem mass spectrometry analysis to quantify proteins of the protein corona (PC) and of A549 cells. Results showed that increasing the concentration of pre-incubated BSA changed the composition ratio of the proteins of the PCs adsorbed on the MWCNTs and also decreased the cellular uptake of A549 cells treated with BSA-coated MWCNTs. In the proteomic analysis, proteins related to the ribosome, ribosome biogenesis in eukaryotes, and oxidative phosphorylation pathways were downregulated in the low concentration BSA-treated cell groups, while proteins related to the mRNA surveillance pathway were downregulated in the high-BSA-concentration cell groups. Moreover, circular dichroism and Fourier-transform infrared spectroscopy results showed that the α-helix structure, a secondary structure of the adsorbed proteins, increased as a function of BSA concentration. Taken together, BSA concentration affects the adsorption and secondary structure of PCs on MWCNTs, which in turns affect their intracellular behavior. Our results may provide insight for the design of modification methods to increase the biocompatibility of MWCNTs.
HostingRepository	PRIDE
AnnounceDate	2024-10-21
AnnouncementXML	Submission_2024-10-21_07:56:42.570.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Young Lee
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-13 08:32:31	ID requested
⏵ 1	2024-10-21 07:56:42	announced

10.1039/D4NA00743C;

submitter keyword: mass spectrometry, quantitative proteomics, albumin,multi-walled carbon nanotubes

Jin Gyeong Son
contact affiliation	Nanobio Measurement Group, Division of Biomedical Metrology, Korea Research Institute of Standard and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea
contact email	yeskyoung@kriss.re.kr
lab head
Sun Young Lee
contact affiliation	Korea Research Institute of Standards and Science
contact email	sylee12@kriss.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052226
     1. Label: PRIDE project
     2. Name: Influence of Albumin Concentration on Surface Characteristics and Cellular Responses in the Pre-Incubation of Multi-Walled Carbon Nanotubes