PXD052190

PXD052190 is an original dataset announced via ProteomeXchange.

Title	The bipolar disorder GWAS risk gene NEK4 modulates circadian fluctuations of murine emotional behaviors and synaptogenesis
Description	Through integrative analyses of GWAS summary statistics, population genetic data and expression quantitative trait loci (eQTL) annotations, we found that elevated NEK4 expression in hippocampus was causatively linked to the risk of BD. To examine the biological impact of this gene, we generated NEK4 conditional knock-in mice in pyramidal neurons of the adult forebrain (NEK4 cKI mice) and NEK4 overexpression mice with adeno-associated virus (AAV) mediated in the dorsal hippocampus (NEK4 OE mice). Since NEK4 encodes a serine/threonine kinase, we explored the phosphoproteomic profiles of these mice at different time of the day and compared them with those of the control mice. We isolated the hippocampal proteins of the NEK4 OE mice and the control mice during day and night respectively (n = 3 for each group), and performed phosphorylated 4D-label-free quantitative proteomics analyses. After stringent quality control, 5376 quantifiable phosphorylated peptides were identified in 2415 phosphorylated proteins
HostingRepository	iProX
AnnounceDate	2024-05-11
AnnouncementXML	Submission_2025-01-05_20:10:36.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao Li
SpeciesList	scientific name: Mus ; NCBI TaxID: 10088;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 19:37:54	ID requested
1	2024-10-15 22:57:35	announced
⏵ 2	2025-01-05 20:10:37	announced	2025-01-06: Add Publication.

Yang ZH, Cai X, Zhang CY, Zhang Q, Li M, Ding ZL, Guo Y, Ma G, Yang CH, Guo L, Chang H, Wang C, Li M, Xiao X, NEK4 modulates circadian fluctuations of emotional behaviors and synaptogenesis in male mice. Nat Commun, 15(1):9180(2024) [pubmed]

submitter keyword: Bipolar disorder, genome-wide association study, circadian fluctuations, emotional behaviors, dendritic spine morphogenesis, synaptogenesis

Ming Li
contact affiliation	Kunming Institute of Zoology Chinese Academy of Sciences
contact email	limingkiz@mail.kiz.ac.cn
lab head
Miao Li
contact affiliation	Kunming Institute of Zoology Chinese Academy of Sciences
contact email	limiao1@mail.kiz.ac.cn
dataset submitter

iProX dataset URI