PXD052189

PXD052189 is an original dataset announced via ProteomeXchange.

Title	Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain
Description	Neuroimmune interactions mediate intercellular communication and underlie critical brain functions. Microglia, CNS-resident macrophages, modulate the brain through direct physical interactions and the secretion of molecules. One such secreted factor, the complement protein C1q, contributes to complement-mediated synapse elimination in development and disease models, yet brain C1q protein levels increase significantly throughout aging. How age-related C1q affects neuronal function has not been demonstrated. Here we report that C1q interacts with neuronal ribonucleoprotein (RNP) complexes in an age-dependent manner. Purified C1q protein undergoes RNA-dependent liquid-liquid phase separation (LLPS) in vitro, and the interaction of C1q with neuronal RNP complexes in vivo is dependent on RNA and endocytosis. Mice lacking C1q have age-specific alterations in neuronal protein synthesis in vivo and impaired fear memory extinction. Together, our findings reveal a biophysical property of C1q that underlies RNA- and age-dependent neuronal interactions and demonstrate a unique role of C1q in critical intracellular neuronal processes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:58.219.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052189
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicole Scott-Hewitt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 18:31:21	ID requested
1	2024-07-01 13:26:10	announced
⏵ 2	2024-10-22 06:47:58	announced	2024-10-22: Updated project metadata.

10.1016/J.CELL.2024.05.058;

10.6019/PXD052189;

submitter keyword: complement, RNA binding protein, RNA granule, C1q,microglia, liquid-liquid phase separation, neuroimmune, ribonucleoprotein complex, translation, polysome

Beth Stevens
contact affiliation	Kirby Neurobiology Center, Boston Children's Hospital, Boston MA
contact email	beth.stevens@childrens.harvard.edu
lab head
Nicole Scott-Hewitt
contact affiliation	Boston Children's Hospital
contact email	nicole.scott-hewitt@childrens.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD052189

PRIDE project URI

• PRIDE
  1. PXD052189
     1. Label: PRIDE project
     2. Name: Microglial-derived C1q integrates into neuronal ribonucleoprotein complexes and impacts protein homeostasis in the aging brain