PXD052180

PXD052180 is an original dataset announced via ProteomeXchange.

Title	Identification of CDKN3 as an independent prognostic marker in LGG correlates with tumor proliferation and immune infiltration
Description	Low-grade glioma (LGG) is a primary, slow-growing brain tumor; however, its treatment and prognosis remain challenging. In this study, we analyzed cancer data from the TCGA database, focusing particularly on the expression of the CDKN3 gene in LGG. The results showed that high CDKN3 expression in LGG patients was significantly associated with poor survival outcomes. Further gene expression analysis revealed that 379 genes were significantly upregulated in LGG samples with high CDKN3 expression, and these genes were primarily involved in the mitotic cell cycle and extracellular matrix organization. Additionally, high CDKN3 expression was closely linked to key signaling pathways such as tumor inflammation, hypoxic response, and tumor proliferation. Immune microenvironment analysis showed that high CDKN3 expression significantly increased the expression of CD4+ T cells and specific immune checkpoint genes, suggesting a potentially poor response to immune checkpoint blockade therapy. Through in vitro and in vivo experiments, we confirmed that CDKN3 silencing significantly inhibited the proliferative capacity of LGG cells. Proteomics revealed that CDKN3 can bind ARG1 and upregulate intracellular arginine concentrations. These findings not only improve our understanding of LGG biology but also provide scientific evidence for developing potential therapeutic strategies targeting CDKN3.
HostingRepository	PRIDE
AnnounceDate	2024-05-10
AnnouncementXML	Submission_2024-05-10_09:23:43.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	pu wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 09:14:59	ID requested
⏵ 1	2024-05-10 09:23:44	announced

Du B, Wang J, Zheng J, Huo J, Wang P, Identification of KIFC1 as an independent prognostic marker in renal clear cell carcinoma correlates with tumor proliferation and immune infiltration. Sci Rep, 13(1):16572(2023) [pubmed]

10.1038/s41598-023-43732-4;

submitter keyword: human A172,CDKN3

pu wang
contact affiliation	changzhi medical college
contact email	372746529@qq.com
lab head
pu wang
contact affiliation	changzhi medical college
contact email	372746529@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052180
     1. Label: PRIDE project
     2. Name: Identification of CDKN3 as an independent prognostic marker in LGG correlates with tumor proliferation and immune infiltration