PXD052178

PXD052178 is an original dataset announced via ProteomeXchange.

Title	Proteomics of E. coli extracellular vesicles
Description	Escherichia coli A0 34/86 (EcO83) is a probiotic bacterium that is used in newborns to prevent nosocomial infections and diarrhoea. Mechanistically, EcO83 enhances innate immunity by inducing key inflammatory cytokines in vitro, and when administered intranasally, it modulates the recruitment of cells to the lung. This study investigates the immunomodulatory effects of EcO83-derived extracellular vesicles (EcO83-EVs) as a safer alternative to live bacteria. We isolated, purified, and characterised EcO83-EVs following the MISEV guidelines. The proteomics data of the EVs were compared with the proteomics data of cell extracts of the EcO83.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_16:02:40.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johan Palmfeldt
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-10 08:15:13	ID requested
⏵ 1	2025-05-06 16:02:41	announced

10.1002/jev2.70004;

Razim A, Zab, ł, ocka A, Schmid A, Thaler M, Č, ern, ý V, Weinmayer T, Whitehead B, Martens A, Skalska M, Morandi M, Schmidt K, Wysmo, ł, ek ME, V, é, gv, á, ri A, Srutkova D, Schwarzer M, Neuninger L, Nejsum P, Hrd, ý J, Palmfeldt J, Brucale M, Valle F, G, ó, rska S, Wisgrill L, Inic-Kanada A, Wiedermann U, Schabussova I, Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells. J Extracell Vesicles, 13(10):e70004(2024) [pubmed]

submitter keyword: extracellular vesicles, E. coli, EcO83, proteome

Johan Palmfeldt
contact affiliation	Aarhus University
contact email	Johan.palmfeldt@clin.au.dk
lab head
Johan Palmfeldt
contact affiliation	Aarhus University Hospital
contact email	johan.palmfeldt@clin.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052178

PRIDE project URI

• PRIDE
  1. PXD052178
     1. Label: PRIDE project
     2. Name: Proteomics of E. coli extracellular vesicles