PXD052150 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chaperonic function of a C/D snoRNA dictates the structure and function of translating ribosomes |
| Description | Leishmania cause cutaneous and visceral diseases affecting millions worldwide. The parasite cycle between insect and mammalian hosts. In this study, we determined the landscape of this 2’-O- methylation (Nm) modification on the rRNA and found that two positions change during cycling between its two life stages. Overexpression of LM32Cs1C1 snoRNA guiding one of these positions compromised growth affecting translation. CryoEM structure of ribosomes from cells overexpressing the snoRNA and its mutant version that cannot guide Nm at ~2.4 Å resolution, suggested no structural changes in small subunit rRNA carrying the Nm modification but changes were observed in H68 of the large subunit rRNA reflecting the function of a second rRNA interaction domain with the snoRNA. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-02 |
| AnnouncementXML | Submission_2026-06-02_01:51:14.069.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tirza Doniger |
| SpeciesList | scientific name: Leishmania major strain Friedlin; NCBI TaxID: NEWT:347515; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-05-09 13:09:35 | ID requested | |
| ⏵ 1 | 2026-06-02 01:51:14 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: cryo-EM, ribosome, chaperon,snoRNA, translation |
Contact List
| Prof Shulamit Michaeli |
|---|
| contact affiliation | The Mina & Everard Goodman Faculty of Life Sciences Bar Ilan University |
| contact email | shulamit.michaeli@biu.ac.il |
| lab head | |
| Tirza Doniger |
|---|
| contact affiliation | Bar-Ilan University |
| contact email | tirza.doniger@biu.ac.il |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD052150 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052150
- Label: PRIDE project
- Name: Chaperonic function of a C/D snoRNA dictates the structure and function of translating ribosomes
