PXD052125

PXD052125 is an original dataset announced via ProteomeXchange.

Title	Chemoenzymatic profiling of multiple glycosylation by a single one-step probe
Description	Protein glycosylation, mainly divided into N-glycosylation and O-glycosylation, is the most prevalent and complex protein posttranslational modification. However, profiling of multiple glycosylation types from a given biological sample is still a challenging issue in glycoscience. Here, a “one-step probe”, directly carried enrichment support, was designed and synthesized. This probe could be accepted by two substrate-specific sialyltransferases to label and enrich N-linked glycans and O-linked glycans in a one-step manner. Combing mass spectrometry (MS) technology, multiple glycoproteomes including N-glycosylation, core fucosylation and truncated mucin-type O-glycosylation can be profiled simultaneously. By this strategy, multiple glycosylation from two breast cancer cell lines including less invasive (MCF7) and highly invasive (MDA-MB-468) were profiled. The results showed that core fucosylation changed significantly while mucin-type O-glycosylation also changed to a lesser extent in two cell lines. Protein-protein interaction (PPI) network analysis showed that the core-fucosylated sites of key genes in ferroptosis, a novel type of iron-dependent cell death, were significantly upregulated in MDA-MB-468. These results indicated that core fucosylation could be a potential biomarker and drug target for highly invasive breast cancer.
HostingRepository	iProX
AnnounceDate	2024-05-09
AnnouncementXML	Submission_2024-05-08_22:15:03.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqiu Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	complex glycosylation
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-08 22:12:01	ID requested
⏵ 1	2024-05-08 22:15:03	announced

Dataset with its publication pending

submitter keyword: N-glycosylation, core fucosylation, O-glycosylation, one-step probe

Hu Zhou
contact affiliation	Shanghai Institute of Materia Medica
contact email	zhouhu@simm.ac.cn
lab head
Yuqiu Wang
contact affiliation	Shanghai Institute of Materia Medica
contact email	995752924@qq.com
dataset submitter

iProX dataset URI