PXD052106

PXD052106 is an original dataset announced via ProteomeXchange.

Title	Exposure to a PFOA, PFOS and PFHxS mixture during gestation and lactation alters the liver proteome in offspring of CD-1 mice
Description	PFAS are persistent man-made chemicals considered to be emerging pollutants, with PFOA, PFOS, and PFHxS having associations with liver toxicity and steatosis. PFOA, PFOS, and PFHxS can undergo placental/lactational transfer, however, little is known about the impact of PFAS mixtures during the developmental window, nor maternal diet on PFAS adverse effects. It was hypothesized that gestational/lactational PFAS exposure would alter the pup liver proteome. The work herein evaluated the liver proteome in offspring, identifying potential biochemical/signaling pathways altered via maternal PFAS exposure. Timed-pregnant CD-1 dams were fed a standard chow or 60% kcal high-fat diet. From GD1 until PND20, dams were orally gavaged daily with either 0.5% Tween 20, individual PFOA, PFOS, PFHxS at 1 mg/kg, or a mixture (1 mg/kg each, totaling 3 mg/kg). Livers were collected from PND21 offspring and SWATH-MS pro-teomics was performed. IPA analysis revealed disease and biological function pathways involved in liver damage, xenobiotics, and lipid regulation were modulated by PFAS exposure in the PND21 liver: lipid transport, storage, oxidation, and synthesis, xenobiotic metabolism and transport, liver damage and inflammation, and fatty acid metabolism, oxidation and transport. This indicates the pup liver proteome is altered via maternal exposure and predisposes the pup to metabolic dysfunctions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:42.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emily Kaye
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-08 02:23:18	ID requested
1	2024-05-09 07:56:29	announced
⏵ 2	2024-10-22 06:38:43	announced	2024-10-22: Updated project metadata.

10.3390/TOXICS12050348;

submitter keyword: PFOA,CD-1 Mouse, PFOS, Maternal exposure, Perinatal, Proteome, Hepatotoxicity, Liver, PFAS Mixture, PFHxS

Angela Slitt
contact affiliation	University of Rhode Island
contact email	angela_slitt@uri.edu
lab head
Emily Kaye
contact affiliation	University of Rhode Island
contact email	emilykaye@uri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052106
     1. Label: PRIDE project
     2. Name: Exposure to a PFOA, PFOS and PFHxS mixture during gestation and lactation alters the liver proteome in offspring of CD-1 mice