PXD052096

PXD052096 is an original dataset announced via ProteomeXchange.

Title	Region-selective and site-specific glycation of influenza proteins surrounding the viral envelope membrane
Description	Analysis of protein modifications is critical for quality control of therapeutic biologics. We used highly sensitive LC MS/MS analyses combined with multiple enzyme digestions to determine low abundance early-stage lysine glycation products of influenza vaccines derived from embryonated chicken eggs and cultured cells. A method utilizing chemoselective labeling of glycated lysine residues, through rapid conversion of site-specific glycation to stable N-ethylmaleimide derivatives at mildly acidic conditions, identified highly reactive lysine sites of proteins. As a result, we determined a widespread distribution of lysine modifications attributed by the region-selectivity and site-specificity of glycation toward influenza matrix 1, hemagglutinin and neuraminidase.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:57:57.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Min She
SpeciesList	scientific name: Influenza A virus (A/turkey/Italy/3466/1999(H7N1)); NCBI TaxID: 1094375;
ModificationList	complex glycosylation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 16:50:43	ID requested
⏵ 1	2024-10-17 05:57:57	announced

She YM, Jia Z, Zhang X, Region-selective and site-specific glycation of influenza proteins surrounding the viral envelope membrane. Sci Rep, 14(1):18975(2024) [pubmed]

10.1038/s41598-024-69793-7;

submitter keyword: influenza vaccine, glycation, mass spectrometry

Xu Zhang
contact affiliation	Health Canada
contact email	xu.zhang@hc-sc.gc.ca
lab head
Yi-Min She
contact affiliation	Centre for Oncology, Radiopharmaceuticals and Research, Biologic and Radiopharmaceutical Drugs Directorate, Health Canada
contact email	yiminshe@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD052096

PRIDE project URI

• PRIDE
  1. PXD052096
     1. Label: PRIDE project
     2. Name: Region-selective and site-specific glycation of influenza proteins surrounding the viral envelope membrane