PXD052066

PXD052066 is an original dataset announced via ProteomeXchange.

Title	Whole-cell proteome analyses of prototrophic Schizosaccharomyces pombe wild-type and mug174Δ strains during vegetative growth and in G0/cellular quiescence (1 day, 7 days, or 14 days following nitrogen depletion) using liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) and the label-free quantitative proteomics method.
Description	Using a whole-cell proteome approach, we investigated the effect of the mug174+ gene deletion on the proteome during vegetative growth and G0/cellular quiescence in the fission yeast Schizosaccahromyces pombe. We revealed that (1) Mug174 ensured the protein expression from intron-containing genes during vegetative growth, and (2) the proteins involved in ribosome biogenesis were increased, whereas some of the G0 essential proteins were decreased in the absence of Mug174. The data from our proteome analyses indicated that Mug174 is essential for proper protein expression.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:15:01.935.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoling Deng
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NCBITaxon:4896; scientific name: Schizosaccharomyces pombe 972h-; NCBI TaxID: 284812;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 03:23:59	ID requested
⏵ 1	2024-10-17 05:15:02	announced

10.1093/nar/gkae463;

Deng X, Yao Q, Horvath A, Jiang Z, Zhao J, Fischer T, Sugiyama T, The fission yeast ortholog of Coilin, Mug174, forms Cajal body-like nuclear condensates and is essential for cellular quiescence. Nucleic Acids Res, 52(15):9174-9192(2024) [pubmed]

submitter keyword: cellular quiescence,Schizosaccharomyces pombe, G0, vegetative growth, LC-MS/MS

Tomoyasu Sugiyama
contact affiliation	School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
contact email	tsugiyama@shanghaitech.edu.cn
lab head
Xiaoling Deng
contact affiliation	ShanghaiTech University
contact email	dengxl@shanghaitech.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD052066

PRIDE project URI

• PRIDE
  1. PXD052066
     1. Label: PRIDE project
     2. Name: Whole-cell proteome analyses of prototrophic Schizosaccharomyces pombe wild-type and mug174Δ strains during vegetative growth and in G0/cellular quiescence (1 day, 7 days, or 14 days following nitrogen depletion) using liquid chromatography followed by tandem mass spectrometry (LC-MS/MS) and the label-free quantitative proteomics method.