PXD052062

PXD052062 is an original dataset announced via ProteomeXchange.

Title	E cadherin appears to be an important on/off switch for initiating bile canaliculi formation
Description	The mechanisms underlying cell polarization are fundamental in biology, yet they are not fully understood. This is particularly true for hepatocytes, which exhibit complex polarization distinct from other epithelial cells, enabling the formation of the bile canaliculi (BCs) network that is responsible for major liver function. To identify key proteins involved in hepatocytes polarization and BC formation, we performed a proteomic approach to compare the human hepatocyte cell line HepG2 to its sub clone HepG2/C3A which, shows much greater efficiency in forming mature BCs. We localized for the first time, LimA1 and Espin at the BC, which could play an important role at this place, and we confirmed the presence of NHE-RF1. Using a protein repression strategy directed against selected proteins, we highlighted the unique role of E cadherin in the initiation of BC formation.
HostingRepository	PRIDE
AnnounceDate	2026-02-09
AnnouncementXML	Submission_2026-02-09_01:10:06.489.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052062
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hélène Diemer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-07 02:42:30	ID requested
⏵ 1	2026-02-09 01:10:07	announced

10.6019/PXD052062;

10.1016/J.IMPACT.2025.100578;

submitter keyword: Human, bile canaliculi, shotgun proteomic, hepatocyte, Liver

Sarah Cianférani
contact affiliation	Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67087 Strasbourg, France
contact email	sarah.cianferani@unistra.fr
lab head
Hélène Diemer
contact affiliation	LSMBO
contact email	hdiemer@unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD052062

PRIDE project URI

• PRIDE
  1. PXD052062
     1. Label: PRIDE project
     2. Name: E cadherin appears to be an important on/off switch for initiating bile canaliculi formation