PXD052032

PXD052032 is an original dataset announced via ProteomeXchange.

Title	Charting the dynamic trophoblast plasma membrane identifies LYN as a functional regulator of syncytialization
Description	The differentiation of placental cytotrophoblasts (CTBs) into the syncytiotrophoblast (STB) layer results in significant remodeling of the plasma membrane proteome. Here, we use a peroxidase-catalyzed proximity labeling strategy to quantitatively map the dynamic plasma membrane proteomes of CTBs and STBs. Coupled with mass spectrometry-based proteomics, we identify hundreds of plasma membrane proteins and observe quantitative changes in protein abundance throughout differentiation, including the upregulation of the plasma membrane-localized non-receptor tyrosine kinase LYN. We show that both siRNA-mediated knockdown and small molecule inhibition of LYN kinase function impairs CTB fusion and abrogates the expression of syncytialization markers, presenting a function for LYN outside of its canonical role in immunological signaling. Our results demonstrate the use of the proximity labeling platform to discover functional regulators within the plasma membrane and provide new avenues to regulate trophoblast differentiation.
HostingRepository	PRIDE
AnnounceDate	2025-10-02
AnnouncementXML	Submission_2025-10-02_12:16:48.550.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052032
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Abigail Reeves
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-06 18:12:45	ID requested
⏵ 1	2025-10-02 12:16:49	announced

10.1021/ACSCHEMBIO.4C00443;

10.6019/PXD052032;

submitter keyword: trophoblast,plasma membrane, proximity labeling, syncytialization

Mia L
contact affiliation	The Scripps Research Institute
contact email	miahuang@scripps.edu
lab head
Abigail Reeves
contact affiliation	The Scripps Research Institute
contact email	areeves@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD052032

PRIDE project URI

• PRIDE
  1. PXD052032
     1. Label: PRIDE project
     2. Name: Charting the dynamic trophoblast plasma membrane identifies LYN as a functional regulator of syncytialization