PXD052016

PXD052016 is an original dataset announced via ProteomeXchange.

Title	A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses
Description	3D tumoroids have revolutionized in vitro/ex vivo cancer biology by recapitulating the complex diversity of tumors. While tumoroid provide new insights into cancer development and treatment response, several limitations remain. The tumor microenvironment, especially the immune system, strongly influences tumor development. Absence of immune cells in tumoroids may yield flawed conclusions. Macrophages, key players in tumor progression, pose integration challenges into tumoroid. In this study, we established three optimized and standardized methods for co-culturing human macrophages with breast cancer tumoroids: a semi-liquid model and two matrix-embedded models tailored for specific applications. Optimizing culture conditions, we tracked interactions and macrophage infiltration using flow cytometry and light sheet microscopy. Macrophages not only influenced tumoroid molecular profiles but also chemotherapy response. This underscores the importance of increasing the complexity of current 3D models to more accurately reflect in vivo conditions. The three models have different application and can be used in various ways.
HostingRepository	PRIDE
AnnounceDate	2024-05-31
AnnouncementXML	Submission_2024-05-31_03:43:14.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonella Raffo Romero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF fleX

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-06 07:07:28	ID requested
⏵ 1	2024-05-31 03:43:14	announced

10.1016/J.CRMETH.2024.100792;

submitter keyword: breast cancer, microscopy,Tumoroids, macrophages, mass spectrometry imaging, drug response

Michel Salzet
contact affiliation	PRISM U1192 Universite de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Antonella Raffo Romero
contact affiliation	PRISM Laboratory - Inserm U1192 Univerty of Lille
contact email	antonella.raffo-romero@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD052016

PRIDE project URI

• PRIDE
  1. PXD052016
     1. Label: PRIDE project
     2. Name: A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses