PXD052013

PXD052013 is an original dataset announced via ProteomeXchange.

Title	Active downregulation of the type III secretion system at higher local cell densities promotes Yersinia replication and dissemination
Description	The T3SS injectisome is used by Gram-negative bacteria, including important pathogens, to manipulate eukaryotic target cells by injecting effector proteins. Some bacterial species display bimodal expression of the T3SS, allowing the T3SS-negative population to benefit from the activity of their T3SS-positive siblings without investing into assembly and production of the injectisome. In contrast, Yersinia enterocolitica, a main T3SS model organism that uses the system to evade the host immune response, was thought to uniformly express and assemble injectisomes, which are then activated by target cell contact. In this study, we found that at higher local bacterial concentrations, Yersinia actively downregulates T3SS expression, assembly and activity. This effect is reversible, highly specific, and distinct from stationary phase adaptation. A key player is the main T3SS transcription factor VirF, which is downregulated at the higher cell densities suppressing T3SS activity and whose in trans expression restores T3SS expression and assembly. Transcript analysis showed this effect is conferred by increased levels of the regulatory RNAs csrBC, which sequester the regulatory protein CsrA and destabilize the virF mRNA. Downregulation of the VirF-dependent adhesin YadA led to a drastic reduction of bacterial cell adhesion. We propose that the phenotype described in this study, active downregulation of cell attachment and T3SS secretion at higher local bacterial densities, is a strategy implemented to favor bacterial replication and dissemination during infection.
HostingRepository	PRIDE
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-05_02:40:51.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timo Glatter
SpeciesList	scientific name: Yersinia enterocolitica LC20; NCBI TaxID: 1443113;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-06 05:49:52	ID requested
⏵ 1	2025-08-05 02:40:51	announced

Dataset with its publication pending

submitter keyword: Yersinia enterocolitica, T3SS injectisome

Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact email	timo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliation	Max Planck Institute for Terrestrial Microbiology Marburg
contact email	timo.glatter@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD052013

PRIDE project URI

• PRIDE
  1. PXD052013
     1. Label: PRIDE project
     2. Name: Active downregulation of the type III secretion system at higher local cell densities promotes Yersinia replication and dissemination