PXD052010

PXD052010 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	CONNETTING ANTI-2GLYCOPROTEIN I ANTIBODIES, NETosis AND ENDOTHELIAL CELL ACTIVATION
Description	This project aims to explore the intricate relationship between anti-β2glycoprotein I (aβ2GPI) antibodies, neutrophil extracellular traps (NETs), and endothelial cell activation in Antiphospholipid Syndrome (APS), an autoimmune disorder characterized by thrombotic events. This study tested the hypothesis that aβ2GPI antibodies may both induce NETosis and act as NET-binding antibodies. It further evaluated the ability of aβ2GPI-induced NET to cause endothelial activation, TF expression increase and endothelial disfunction. Furthermore, a proteomics analysis was conducted to compare aβ2GPI-induced NETs with NETs induced by phorbol myristate acetate (PMA). Please note that aβ2GPI-induced NETs are labeled as 'TV' in the raw files, while PMA-induced NETs are labeled as 'T'.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:16:53.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-06 03:18:07	ID requested
⏵ 1	2025-08-24 16:16:54	announced

Publication List

10.1093/rheumatology/keaf204;
Mancuso S, Caliste M, Petretto A, Corsiero E, Grinovero N, Capozzi A, Riitano G, Barbati C, Truglia S, Alessandri C, Sorice M, Bombardieri M, Conti F, 2glycoprotein I-induced neutrophil extracellular traps cause endothelial activation. Rheumatology (Oxford), 64(8):4796-4805(2025) [pubmed]

10.1093/rheumatology/keaf204;

Mancuso S, Caliste M, Petretto A, Corsiero E, Grinovero N, Capozzi A, Riitano G, Barbati C, Truglia S, Alessandri C, Sorice M, Bombardieri M, Conti F, 2glycoprotein I-induced neutrophil extracellular traps cause endothelial activation. Rheumatology (Oxford), 64(8):4796-4805(2025) [pubmed]

Keyword List

submitter keyword: NETosis, autoimmune disorder, Antiphospholipid Syndrome, ENDOTHELIAL CELL ACTIVATION

submitter keyword: NETosis, autoimmune disorder, Antiphospholipid Syndrome, ENDOTHELIAL CELL ACTIVATION

Contact List

Andrea Petretto
contact affiliation	IRCCS G. Gaslini, Core Facilities - Clinical Proteomics and Metabolomics, Genoa, Italy
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD052010
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD052010

PRIDE project URI

Repository Record List

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