PXD051983

PXD051983 is an original dataset announced via ProteomeXchange.

Title	Lost in translation? Proteomic evidence for a muted molecular response to thermal stress in a stenothermal Antarctic fish and possible evolutionary mechanisms
Description	Antarctic notothenioid fishes are noteworthy for their history of isolation and indications they lack the heat shock response. The mechanistic basis for stenothermy has not been fully elucidated, and some aspects of stenothermy could arise post-transcriptionally. Antarctic emerald rockcod (Trematomus bernacchii) were sampled after exposure to chronic and/or acute high temperatures, followed by assessment of proteomic responses in brain, gill, and kidney using tissue-specific DIA assay libraries. Few cellular stress response proteins were induced, and overall responses were modest in terms of numbers of differentially expressed proteins and their fold changes. Inconsistencies in protein induction across treatments and tissues are suggestive of dysregulation, rather than an adaptive response. Changes in regulation of translation in Antarctic notothenioids could explain these patterns. Some components of the “integrative stress response” that regulates translation are highly conserved (e.g., Ser-52 of eIF2α), but the eIF2α kinases GCN2 and PERK may have evolved along different trajectories in Antarctic fishes. Together, these observations suggest a novel hypothesis for stenothermy and the absence of a coordinated cellular stress response in Antarctic fishes.
HostingRepository	PanoramaPublic
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_10:28:23.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Trematomus bernacchii; NCBI TaxID: 40690;
ModificationList	Acetyl; Carbamidomethyl; Oxidation
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 23:35:28	ID requested
⏵ 1	2024-10-03 10:28:24	announced

Dowd WW, K, ü, ltz D, Lost in translation? Evidence for a muted proteomic response to thermal stress in a stenothermal Antarctic fish and possible evolutionary mechanisms. Physiol Genomics, 56(11):721-740(2024) [pubmed]

submitter keyword: Ecological proteomics, Thermal stress, Cellular stress response, Heat shock response, Integrative stress response, DIA, Quantitative proteomics, Stenothermy

Dietmar Kültz
contact affiliation	University of California, Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	University of California, Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI