PXD051981

PXD051981 is an original dataset announced via ProteomeXchange.

Title	KDR-dependent tyrosine phosphorylation in HTLV-1 transformed cells
Description	Human T-cell leukemia virus type 1 (HTLV-1) is linked to the development of adult T-cell leukemia (ATL) and the neuroinflammatory disease, HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 Tax oncoprotein regulates viral gene expression and the NF-kB pathway to promote the survival of HTLV-1 infected T cells. In thsi study, we utilize a kinome-wide shRNA screen to identify the tyrosine kinase KDR/VEGFR2 as an essential survival factor of HTLV-1-transformed T cells. Inhibition of KDR induces apoptosis of Tax expressing HTLV-1-transformed cell lines and CD4+ T cells from HAM/TSP patients. Phosphoproteomics analysis of HTLV-1 transformed cells treated with a KDR inhibitor revealed inhibition of the phosphorylation of multiple receptors/cell surface proteins, ubiquitin conjugating systems, proteases, phosphatases, apoptotic regulatory factors, adhesion/extracellular matrix proteins and viral proteins. This work suggests that HTLV-1 Tax has hijacked KDR kinase activity to promote Tax stability and the proliferation and survival of HTLV-1 infected cells.
HostingRepository	PRIDE
AnnounceDate	2024-06-14
AnnouncementXML	Submission_2024-06-14_03:04:03.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edward Harhaj
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 17:30:47	ID requested
⏵ 1	2024-06-14 03:04:03	announced

Dataset with its publication pending

submitter keyword: Tax,KDR, VEGFR2, HTLV-1

Edward Harhaj
contact affiliation	Penn State College of Medicine
contact email	ewh110@psu.edu
lab head
Edward Harhaj
contact affiliation	Penn State College of Medicine
contact email	ewh110@psu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051981
     1. Label: PRIDE project
     2. Name: KDR-dependent tyrosine phosphorylation in HTLV-1 transformed cells