PXD051970

PXD051970 is an original dataset announced via ProteomeXchange.

Title	A systems biology-oriented investigation of Arabidopsis retrograde-signaling-defective mutant gun1 reveals the co-expression-based HUBs at the center of plastid development and homeostasis
Description	In the present study we investigated at the system level the proteome obtained from seedlings of Arabidopsis thaliana wild-type Col-0 and gun1-101 and gun1-102 mutant plants treated or not with lincomycin (Lin), a chloroplast translation inhibitor widely used to inhibit chloroplast biogenesis and to trigger the plastid-to-nucleus retrograde communication. Specifically, we attempted a description of cellular responses to Lincomycin in wild-type genetic background and in retrograde signaling-defective mutant gun1. Hypotheses and key players emerged by our holistic view were validated at the experimental level. Our findings suggest that, in the presence of Lincomycin, cell responses rely on the plastid compartment in a GUN1-dependent manner, while in the gun1-mutant background, the activity of extra-plastid compartments prevails. Moreover, results obtained by complementary approaches depict the Oxygen Evolving Complex subunit PsbO as an atypical Photosynthesis-related protein by accumulating in non-photosynthetic plastids and having a superhub role in modulating chloroplast dismantling upon impairment of plastid functions.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:10:13.977.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milena Marsoni
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 07:50:37	ID requested
⏵ 1	2025-07-13 16:10:14	announced

Di Silvestre D, Jeran N, Domingo G, Vannini C, Marsoni M, Fortunato S, de Pinto MC, Tamborrino A, Negroni YL, Zottini M, Hong LT, Lomagno A, Mauri P, Pesaresi P, Tadini L, A Holistic Investigation of Arabidopsis Proteomes Altered in Chloroplast Biogenesis and Retrograde Signalling Identifies PsbO as a Key Regulator of Chloroplast Quality Control. Plant Cell Environ, 48(8):6373-6396(2025) [pubmed]

10.1111/pce.15611;

submitter keyword: GUN1, PsbO, Hub, Lincomycin

Guido Domingo
contact affiliation	University of Insubria
contact email	g.domingo@uninsubria.it
lab head
Milena Marsoni
contact affiliation	University of Insubria
contact email	milena.marsoni@uninsubria.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051970

PRIDE project URI

• PRIDE
  1. PXD051970
     1. Label: PRIDE project
     2. Name: A systems biology-oriented investigation of Arabidopsis retrograde-signaling-defective mutant gun1 reveals the co-expression-based HUBs at the center of plastid development and homeostasis