PXD051967

PXD051967 is an original dataset announced via ProteomeXchange.

Title	Identification of ADP-ribosylation targets in living HeLa cells using cell-permeable NAD+ probes
Description	Posttranslational modifications of proteins (PTMs) greatly enhance their functional diversity, surpassing the number of gene-encoded variations. One intriguing PTM is ADP-ribosylation, which utilizes nicotinamide adenine dinucleotide (NAD+) as a substrate and is essential in cell signaling pathways regulating cellular responses. Here, we report the first cell-permeable NAD+ analogs that are modified with a dye or an affinity tag, respectively, and demonstrate their suitability as tools for investigating of the cellular ADP-ribosylation process. Utilizing desthiobiotin-tagged probes, we characterized ADP-ribosylome changes during oxidative stress in HeLa cells. We identified proteins previously described as ADP-ribosylation targets or closely associated with them. Therefore, we believe that our cell-permeable NAD+ probes offer reliable tools for comprehensive ADP-ribosylation investigations and understanding cellular responses to stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:19.336.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renata Kasprzyk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 06:44:33	ID requested
1	2024-10-11 08:43:18	announced
⏵ 2	2024-10-22 07:01:20	announced	2024-10-22: Updated project metadata.

Kasprzyk R, Rieth S, Heid P, Stengel F, Marx A, Cell-Permeable Nicotinamide Adenine Dinucleotides for Exploration of Cellular Protein ADP-Ribosylation. Angew Chem Int Ed Engl, 63(51):e202411203(2024) [pubmed]

10.1002/anie.202411203;

submitter keyword: affinity enrichment, HeLa cells, NAD+, cell-permeable probes,ADP-ribosylation

Andreas Marx
contact affiliation	Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Germany
contact email	andreas.marx@uni-konstanz.de
lab head
Renata Kasprzyk
contact affiliation	University of Konstanz
contact email	renata.kasprzyk@uni-konstanz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD051967

PRIDE project URI

• PRIDE
  1. PXD051967
     1. Label: PRIDE project
     2. Name: Identification of ADP-ribosylation targets in living HeLa cells using cell-permeable NAD+ probes