PXD051961

PXD051961 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of the Skp1 and Cullin1 interactome in Leishmania infantum
Description	Leishmania infantum is the causative agent of visceral leishmaniasis in Latin America, a lethal disease if misdiagnosed or left untreated. The ubiquitin proteasome system (UPS) is the main regulator of intracellular proteolysis in eukaryotes and is required for parasite's to life cycle and infection. E3 ubiquitin ligases play important role in UPS and Cullin-1-RING ligases (CRL1) are the largest and most researched family of E3 in eukaryotes. CRL1 complex is made up of SKP1, Cullin1, RBX1, and F-box proteins. This complex is poorly studied in Leishmania. We investigated the interactome of CRL1 complex in L. infantum combining in vitro and in cellulo experiments to identify the interactome of Cullin1 and SKP1 through affinity purification followed by mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:57.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Felipe Roberti Teixeira
SpeciesList	scientific name: Leishmania infantum; NCBI TaxID: 5671;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-03 05:07:35	ID requested
1	2024-07-01 13:02:33	announced
⏵ 2	2024-10-22 06:47:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: AP-MS,Cullin-1-RING ubiquitin ligases (CRL1), E3 ubiquitin ligases, Leishmania infantum

Felipe Roberti Teixeira
contact affiliation	Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, Brazil.
contact email	frt@ufscar.br
lab head
Felipe Roberti Teixeira
contact affiliation	Universidade Federal de São Carlos (UFSCar)
contact email	lbqc@ufscar.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051961

PRIDE project URI

• PRIDE
  1. PXD051961
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of the Skp1 and Cullin1 interactome in Leishmania infantum