PXD051952

PXD051952 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identifying Cilary Proteins in Bovine Retinas using a Gentile Extraction Method
Description	Mutations in retinal primary cilia are responsible for human blindness but the mechanisms are not fully understood. Characterizing the proteome of an organelle such as cilia, is a fruitful way to understand its function but methods often require considerable sample quantities. Here we develop a method to isolate the primary cilia of photoreceptor cells from bovine retinas. Through LC/MS/MS proteomics analysis we identify proteins enriched for cilia function including ciliopathy disease. This study shows our method can be used to isolate retinal primary cilia to obtain sufficient quantities of native protein samples.
HostingRepository	PRIDE
AnnounceDate	2024-07-11
AnnouncementXML	Submission_2024-07-11_13:45:10.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kevin Drew
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-02 17:06:26	ID requested
⏵ 1	2024-07-11 13:45:11	announced

Publication List

10.17912/micropub.biology.001218;
Fredrick AS, Claussen ER, Fischer SN, Balasanyants S, Rajaraman A, Rosner AC, Drew K, Identifying Ciliary Proteins in Mammalian Retinas using a Gentle Extraction Method. MicroPubl Biol, 2024():(2024) [pubmed]

10.17912/micropub.biology.001218;

Fredrick AS, Claussen ER, Fischer SN, Balasanyants S, Rajaraman A, Rosner AC, Drew K, Identifying Ciliary Proteins in Mammalian Retinas using a Gentle Extraction Method. MicroPubl Biol, 2024():(2024) [pubmed]

Keyword List

submitter keyword: Bovine, rod outer segment, photoreceptor, retina, primary cilia

submitter keyword: Bovine, rod outer segment, photoreceptor, retina, primary cilia

Contact List

Kevin Drew
contact affiliation	Department of Biological Sciences, University of Illinois at Chicago
contact email	ksdrew@uic.edu
lab head
Kevin Drew
contact affiliation	Department of Biological Sciences, University of Illinois at Chicago
contact email	ksdrew@uic.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051952
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051952

PRIDE project URI

Repository Record List

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