PXD051943

PXD051943 is an original dataset announced via ProteomeXchange.

Title	Comparison of the proteome of adult neural stem/progenitor cells (NSPCs) from tdTom-Plin2 heterozygous and wildtype control mice. These data are presented in the manuscript entitled “A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain” by Madsen et al. 2024.
Description	Lipid droplets (LDs) are dynamic lipid storage organelles. They are tightly linked to metabolism and can exert protective functions, making them important players in health and disease. Most LD studies in vivo rely on staining methods, providing only a snapshot. We therefore developed a LD-reporter mouse by endogenously labelling the LD coat protein perilipin 2 (PLIN2) with tdTomato, enabling staining-free fluorescent LD visualisation in living and fixed tissues and cells. We validate this model under standard and high-fat diet conditions and demonstrate that LDs are highly abundant in various cell types in the healthy brain, including neurons, astrocytes, ependymal cells, neural stem/progenitor cells and microglia. Furthermore, we also show that LDs are abundant during brain development and can be visualized using live-imaging of embryonic slices. Taken together, our tdTom-Plin2 mouse serves as a novel tool to study LDs and their dynamics under both physiological and diseased conditions in all tissues expressing Plin2. The proteomic comparison in neural stem/progenitor cells served as a further validation that the fluorescent tag does not alter the LD machinery.
HostingRepository	PRIDE
AnnounceDate	2024-06-11
AnnouncementXML	Submission_2024-06-11_08:13:31.589.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlen Knobloch
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-05-02 06:17:05	ID requested
⏵ 1	2024-06-11 08:13:31	announced

10.1038/S41467-024-49449-W;

submitter keyword: Neural stem/progenitor cells,Lipid droplets, Endogenous tagging of Plin2, LD reporter mouse line

Marlen Knobloch
contact affiliation	University of Lausanne, Department of Biomedical Sciences, Rue du Bugnon 7, 1005 Lausanne
contact email	marlen.knobloch@unil.ch
lab head
Marlen Knobloch
contact affiliation	University of Lausanne, Faculty of Biology and Medicine, Department of Biomedical Sciences
contact email	marlen.knobloch@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD051943
     1. Label: PRIDE project
     2. Name: Comparison of the proteome of adult neural stem/progenitor cells (NSPCs) from tdTom-Plin2 heterozygous and wildtype control mice. These data are presented in the manuscript entitled “A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain” by Madsen et al. 2024.