PXD051895

PXD051895 is an original dataset announced via ProteomeXchange.

Title	Comparative exo-proteomic analysis of Trichoderma antagonists induced by oomycete mycelium highlights diverse antagonism strategies
Description	Plant-beneficial fungi from the genus Trichoderma (Hypocreales, Ascomycota) can control oomyceteous plant-pathogenic Pythium myriotylum (Peronosporales, Oomycota) and thus serve as bioeffectors for the eco-friendly products of crop protection. However, the underlying mechanisms of microbe-microbe interactions have yet to be fully understood. In this study, we focused on the role of the Trichoderma secretome induced by P. myriotylum mycelia. For this purpose, we selected strains showing strong (T. asperellum, T. atroviride, T. virens), moderate (T. cf. guizhouense, T. reesei), and weak (T. parepimyces) activities, respectively, and cultured with the sterilized P. myriotylum mycelia. Secreted proteins were analyzed using label-free LC-MS/MS, bioinformatic localization prediction, gene ontology (GO) annotation, and ortholog analysis. The exoproteomic analysis quantified proteins in the six Trichoderma spp., suggesting unequal antagonistic mechanisms among the strong and weak strains, respectively, with different proportions of putative cellulases, proteases, redox enzymes, and extracellular proteins of unknown function. Notably, proteolysis-related proteins were abundant, while the abundant proteases tended not to be conserved across the species (i.e., non-orthologous). Putative cellobiohydrolases were detected abundantly in all Trichoderma species except for the weak antagonist T. parepimyces, even though its genome encodes for these proteins. Notably, secretomes of the most potent anti-Pythium bioeffectors tended to have higher endo-cellulase activity. Cellulose and other glucans are major components of the oomycete cell wall, which was partly reflected in the cellulases produced by the Trichoderma species. The varying abundances of orthologous proteins suggested the evolution of differing transcription regulation mechanisms across the Trichoderma genus in response to the ubiquitous presence of Oomycota.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:52.608.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paul Daly
SpeciesList	scientific name: Trichoderma atroviride IMI 206040; NCBI TaxID: 452589; scientific name: Trichoderma asperellum CBS 433.97; NCBI TaxID: 1042311; scientific name: Trichoderma virens Gv29-8; NCBI TaxID: 413071; scientific name: Hypocrea jecorina (strain QM6a) (Trichoderma reesei); NCBI TaxID: 431241; scientific name: Trichoderma parepimyces; NCBI TaxID: 692672; scientific name: Trichoderma guizhouense; NCBI TaxID: 1491466;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 10:12:01	ID requested
1	2024-07-18 22:12:33	announced
⏵ 2	2024-10-22 06:50:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Trichoderma, exo-proteins, hydrolyase, antagonism

Paul Daly
contact affiliation	Jiangsu Academy of Agricultural Sciences
contact email	paul4594@hotmail.co.uk
lab head
Paul Daly
contact affiliation	Jiangsu Academy of Agricultural Sciences
contact email	paul4594@hotmail.co.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD051895

PRIDE project URI

• PRIDE
  1. PXD051895
     1. Label: PRIDE project
     2. Name: Comparative exo-proteomic analysis of Trichoderma antagonists induced by oomycete mycelium highlights diverse antagonism strategies