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PXD051891

PXD051891 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
DescriptionDeciphering the intricate tumor-immune interactions within the microenvironment is crucial for advancing cancer immunotherapy. Here, we developed a novel approach integrating highly multiplexed imaging, laser microdissection, and deep visual proteomics to spatially profile the proteomes of 21 distinct cell populations in human colorectal and tonsil cancers with high sensitivity. In colorectal tumors, we uncovered an immunosuppressive macrophage barrier impeding T cell infiltration and regionally altering lymphocyte proteomes. Spatial proteomic analysis revealed distinct functional states of T cells in different tumor compartments. In tonsil cancer, we identified significant tumor cell proteomic heterogeneity influenced by proximity to specific T cell subtypes. Tumor-infiltrating T cells exhibited metabolic adaptations and enhanced stress resilience, enabling migration into hypoxic regions. Our spatially-resolved, highly multiplexed strategy provides a powerful tool to decipher the complex cellular interplay within the tumor microenvironment, with implications for identifying new immunotherapy targets and signatures.
HostingRepositoryPRIDE
AnnounceDate2024-09-16
AnnouncementXMLSubmission_2024-09-16_07:49:36.492.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF SCP; Orbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-04-30 08:32:11ID requested
12024-09-16 07:49:36announced
Publication List
10.1101/2024.05.22.595266;
Keyword List
submitter keyword: Multiplex staining
deep visual proteomics
tumor microenvironment
Contact List
Matthias Mann
contact affiliationNovo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark. Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
contact emailmatthias.mann@cpr.ku.dk
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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