PXD051891

PXD051891 is an original dataset announced via ProteomeXchange.

Title	Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Description	Deciphering the intricate tumor-immune interactions within the microenvironment is crucial for advancing cancer immunotherapy. Here, we developed a novel approach integrating highly multiplexed imaging, laser microdissection, and deep visual proteomics to spatially profile the proteomes of 21 distinct cell populations in human colorectal and tonsil cancers with high sensitivity. In colorectal tumors, we uncovered an immunosuppressive macrophage barrier impeding T cell infiltration and regionally altering lymphocyte proteomes. Spatial proteomic analysis revealed distinct functional states of T cells in different tumor compartments. In tonsil cancer, we identified significant tumor cell proteomic heterogeneity influenced by proximity to specific T cell subtypes. Tumor-infiltrating T cells exhibited metabolic adaptations and enhanced stress resilience, enabling migration into hypoxic regions. Our spatially-resolved, highly multiplexed strategy provides a powerful tool to decipher the complex cellular interplay within the tumor microenvironment, with implications for identifying new immunotherapy targets and signatures.
HostingRepository	PRIDE
AnnounceDate	2024-09-16
AnnouncementXML	Submission_2024-09-16_07:49:36.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mario Oroshi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF SCP; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 08:32:11	ID requested
⏵ 1	2024-09-16 07:49:36	announced

10.1101/2024.05.22.595266;

submitter keyword: Multiplex staining

deep visual proteomics

tumor microenvironment

Matthias Mann
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark. Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
contact email	matthias.mann@cpr.ku.dk
lab head
Mario Oroshi
contact affiliation	Proteomics
contact email	oroshi@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD051891

PRIDE project URI

• PRIDE
  1. PXD051891
     1. Label: PRIDE project
     2. Name: Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics