PXD051887

PXD051887 is an original dataset announced via ProteomeXchange.

Title	A-to-I mRNA editing enables bacteria to produce two protein versions from a single gene and affects bacterial growth
Description	Adenosine-to-inosine (A-to-I) mRNA editing can affect the sequence and function of translated proteins because the ribosome reads inosine (I) as guanosine (G). Previously, we discovered that A-to-I mRNA editing occurs in Escherichia coli. However, the prevalence of A-to-I mRNA editing across bacteria and its biological significance are still unknown. Here, we show that A-to-I mRNA editing occurs in hundreds of genes across dozens of bacterial species. We find that editing events occur within and require a conserved sequence motif. Notably, most editing events (80%) are predicted to recode protein sequences. Indeed, by mass spectrometry, we show for the first time that A-to-I mRNA editing enables bacteria to produce two versions of a protein from a single gene. Finally, we find that perturbing mRNA editing in Acinetobacter baylyi affects bacterial growth, especially at high temperatures. Overall, our work reveals that A-to-I mRNA editing is widespread and can impact bacterial physiology, likely by modulating protein function
HostingRepository	PRIDE
AnnounceDate	2025-07-21
AnnouncementXML	Submission_2025-07-20_16:09:47.163.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dganit Melamed Kadosh
SpeciesList	scientific name: Acinetobacter baylyi; NCBI TaxID: 202950;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-30 04:31:18	ID requested
⏵ 1	2025-07-20 16:09:48	announced

Elias E, Gifford I, Didi L, Fargeon O, Arad D, Cohen-Pavon R, Sorek G, Levin L, Melamed D, Aspit L, Barrick JE, Bar-Yaacov D, A-to-I mRNA editing recodes hundreds of genes in dozens of species and produces endogenous protein isoforms in bacteria. Nucleic Acids Res, 53(13):(2025) [pubmed]

10.1093/nar/gkaf656;

submitter keyword: Adenosine-to-inosine (A-to-I) mRNA editing

gammaproteobacteria

Dan Bar Yaacov
contact affiliation	The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
contact email	danbary@bgu.ac.il
lab head
Dganit Melamed Kadosh
contact affiliation	Technion
contact email	sdganit@tx.technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD051887

PRIDE project URI

• PRIDE
  1. PXD051887
     1. Label: PRIDE project
     2. Name: A-to-I mRNA editing enables bacteria to produce two protein versions from a single gene and affects bacterial growth