PXD051849

PXD051849 is an original dataset announced via ProteomeXchange.

Title	Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression
Description	Post-transcriptional regulation of immune-related transcripts by RNA-binding proteins (RBPs) impacts immune cell responses, including mast cell functionality. Despite their importance in immune regulation, the functional role of most RBPs remains to be understood. By manipulating the expression of specific RBPs in murine mast cells, coupled with mass spectrometry and transcriptomic analyses, we found that the Regnase family of proteins acts as a potent regulator of mast cell physiology. Specifically, Regnase-1 is required to maintain basic cell proliferation and survival, while both Regnase-1 and -3 cooperatively regulate the expression of inflammatory transcripts upon activation, with Tnf being a primary target in both human and mouse cells. Further, Regnase-3 directly interacts with Regnase-1 in mast cells and is necessary to restrain Regnase-1 expression through the destabilization of its transcript. Overall, our study identifies protein interactors of endogenously expressed Regnase factors, characterizes the regulatory interplay between Regnase family members in mast cells, and establishes their role in the control of mast cell homeostasis and inflammatory responses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:43:30.249.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matteo Pecoraro
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-04-29 10:31:55	ID requested
1	2024-05-31 10:21:13	announced
⏵ 2	2024-10-22 06:43:30	announced	2024-10-22: Updated project metadata.

10.26508/LSA.202402784;

submitter keyword: TNF, inflammation, Regnase,Mast cells, RNA-binding proteins, proliferation

Silvia Monticelli
contact affiliation	Institute for Research in Biomedicine, Università della Svizzera italiana, Via Francesco Chiesa 6, CH6500 Bellinzona, Switzerland
contact email	silvia.monticelli@irb.usi.ch
lab head
Matteo Pecoraro
contact affiliation	Institute for Research in Biomedicine, Via Francesco Chiesa 5 - CH-6500 Bellinzona
contact email	matteo.pecoraro@irb.usi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD051849

PRIDE project URI

• PRIDE
  1. PXD051849
     1. Label: PRIDE project
     2. Name: Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression